PUBLICATION

The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo

Authors
Schulte-Merker, S., Ho, R.K., Herrmann, B.G., and Nüsslein-Volhard, C.
ID
ZDB-PUB-961014-1008
Date
1992
Source
Development (Cambridge, England)   116: 1021-1032 (Journal)
Registered Authors
Ho, Robert K., Nüsslein-Volhard, Christiane, Schulte-Merker, Stefan
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Embryonic Induction/genetics*
  • Gene Expression/physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Mesoderm/physiology*
  • Mice
  • Molecular Sequence Data
  • Notochord/physiology*
  • Nuclear Proteins/genetics*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed
1295726
Abstract
Embryos mutant for the T gene, in mice, make insufficient mesoderm and fail to develop a notochord. We report the cloning and sequencing of the T gene in the zebrafish (Brachydanio rerio) and show the nuclear localization of the protein product. Both RNA and protein are found in cells of the germ ring, including enveloping layer cells, prior to and during gastrulation of zebrafish embryos. Nuclei of the yolk syncytial layer do not express Zf-T. High levels of expression are maintained throughout early development in the notochord, while in paraxial mesoderm cells the gene is turned off during gastrulation. Exposure of animal cap cells to activinA induces Zf-T expression, as does transplantation into the germ ring.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes