PUBLICATION

prdm1a drives a fate switch between hair cells of different mechanosensory organs

Authors
Sandler, J.E., Tsai, Y.Y., Chen, S., Sabin, L., Lush, M.E., Sur, A., Ellis, E., Tran, N.T.T., Cook, M., Scott, A.R., Kniss, J.S., Farrell, J.A., Piotrowski, T.
ID
ZDB-PUB-250819-1
Date
2025
Source
Nature communications   16: 76627662 (Journal)
Registered Authors
Farrell, Jeffrey, Kniss, Jonathan, Lush, Mark E., Piotrowski, Tatjana
Keywords
none
Datasets
GEO:GSE268538
MeSH Terms
  • Mutation
  • Positive Regulatory Domain I-Binding Factor 1*/genetics
  • Positive Regulatory Domain I-Binding Factor 1*/metabolism
  • Regeneration
  • Hair Cells, Auditory*/cytology
  • Hair Cells, Auditory*/metabolism
  • Animals
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Cell Differentiation/genetics
  • Lateral Line System*/cytology
  • Lateral Line System*/metabolism
  • Zebrafish/genetics
  • Gene Expression Regulation, Developmental
  • Gene Regulatory Networks
PubMed
40825768 Full text @ Nat. Commun.
Abstract
Vertebrate inner ear mechanosensory hair cells detect sound and gravitational forces. Additionally, fishes have homologous lateral line hair cells in the skin that detect water vibrations for orientation and predator avoidance. Hair cells in the lateral line and ear of fishes and other non-mammalian vertebrates regenerate readily after damage, but mammalians lack this ability, causing deafness and vestibular defects. As yet, experimental attempts at hair cell regeneration in mice result in incompletely differentiated and immature hair cells. Despite differences in regeneration capabilities, the gene regulatory networks (GRNs) driving hair cell maturation during development are highly similar across vertebrates. Here, we show that the transcription factor prdm1a plays a key role in the hair cell fate GRN in the zebrafish lateral line. Mutating prdm1a respecifies lateral line hair cells into ear hair cells, altering morphology and transcriptome. Understanding how transcription factors control diverse hair cell fates in zebrafish is crucial for understanding the yet unsolved regeneration of diverse hair cells in mammalian ears to restore hearing and balance.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping