PUBLICATION
Conjugation of Pea Peptides and D-Xylose via Maillard Glycosylation and Its Functionality to Antagonize Alcohol-Induced Liver Injury in Zebrafish
- Authors
- Li, G., Liu, X., Diao, S., Zheng, X.
- ID
- ZDB-PUB-250814-16
- Date
- 2025
- Source
- Nutrients 17: (Journal)
- Registered Authors
- Keywords
- Maillard glycosylation, antagonize alcohol-induced liver injury, pea glycopeptides, pea peptides
- MeSH Terms
-
- Peptides*/chemistry
- Peptides*/pharmacology
- Liver/drug effects
- Liver/metabolism
- Liver/pathology
- Protein Hydrolysates/chemistry
- Protein Hydrolysates/pharmacology
- Liver Diseases, Alcoholic*/metabolism
- Liver Diseases, Alcoholic*/prevention & control
- Pisum sativum*/chemistry
- Animals
- Malondialdehyde/metabolism
- Zebrafish
- Disease Models, Animal
- Xylose*/chemistry
- Xylose*/pharmacology
- Antioxidants/pharmacology
- Ethanol
- Maillard Reaction
- Pea Proteins*/chemistry
- Pea Proteins*/pharmacology
- Glycosylation
- Oxidative Stress/drug effects
- PubMed
- 40806154 Full text @ Nutrients
Citation
Li, G., Liu, X., Diao, S., Zheng, X. (2025) Conjugation of Pea Peptides and D-Xylose via Maillard Glycosylation and Its Functionality to Antagonize Alcohol-Induced Liver Injury in Zebrafish. Nutrients. 17:.
Abstract
Background In this study, the preparation of pea glycopeptides based on the Maillard glycosylation pathway (PPH-M) and its antagonistic mechanism against alcoholic liver injury in zebrafish were studied.
Results The results showed that the conjugation of D-xylose significantly improved the antioxidant activity of pea protein hydrolysates (PPHs). The structural characterization indicated that PPH was successfully covalent binding to D-xylose, which was mainly manifested as a stretching vibration change in Fourier transform infrared spectroscopy (FTIR) and molecular size increase. Scanning electron microscopy (SEM) and zeta potential also confirmed the covalently bound of the two. In addition, a model of alcohol-induced liver injury in zebrafish was established. Through the intervention of different doses of PPH-M, it was found that the intervention of PPH-M could significantly increase superoxide dismutase (SOD) activity, reduce malondialdehyde (MDA) content, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activity, and significantly improve alcohol-induced liver injury in zebrafish. The protective effect of PPH-M was also confirmed by liver pathology and fluorescence microscopy. Finally, reverse transcription-polymerase chain reaction (qRT-PCR) results indicated that PPH-M could significantly regulate the expression level of antioxidant-related mRNA. PPH-M could also regulate the expression of the Keap1/Nrf2 signaling pathway and up-regulated glutathione synthesis signaling pathway to antagonize alcohol-induced liver injury in zebrafish.
Conclusion This study revealed the mechanism of PPH-M antagonized alcoholic liver injury and laid a theoretical foundation for its development as functional foods.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping