PUBLICATION
Anti-Thrombotic Effects of Coprinus comatus Fibrinolytic Enzyme in Zebrafish
- Authors
- Jing, Y., Wang, J., He, Y., Liu, Z., Liu, X.
- ID
- ZDB-PUB-250730-14
- Date
- 2025
- Source
- Nutrients 17: (Journal)
- Registered Authors
- Keywords
- Coprinus comatus, fibrinolytic enzyme, thrombolysis and anticoagulation
- MeSH Terms
-
- Zebrafish
- Fibrinolytic Agents*/pharmacology
- Phenylhydrazines
- Blood Coagulation/drug effects
- Coprinus*/enzymology
- Disease Models, Animal
- Platelet Activation/drug effects
- Animals
- Thrombosis*/chemically induced
- Thrombosis*/drug therapy
- Fibrinolysis*/drug effects
- PubMed
- 40732981 Full text @ Nutrients
Citation
Jing, Y., Wang, J., He, Y., Liu, Z., Liu, X. (2025) Anti-Thrombotic Effects of Coprinus comatus Fibrinolytic Enzyme in Zebrafish. Nutrients. 17:.
Abstract
Objectives This study investigated the antithrombotic properties of a fibrinolytic enzyme (CFE) purified from the culture supernatant of Coprinus comatus using a zebrafish thrombosis model.
Methods A phenylhydrazine-induced thrombosis model was employed to evaluate the in vivo thrombolytic efficacy and mechanisms of CFE.
Results CFE significantly attenuated thrombogenesis by inhibiting erythrocyte aggregation in the caudal vessels, reducing staining intensity (3.61-fold decrease) and staining area (3.89-fold decrease). Concurrently, CFE enhanced cardiac hemodynamics, increasing erythrocyte staining intensity (9.29-fold) and staining area (5.55-fold) while achieving an 85.19% thrombosis inhibition rate. Behavioral analysis confirmed improved motility, with CFE-treated zebrafish exhibiting 2.23-fold increases in total movement distance and average speed, alongside a 3.59-fold extension in active movement duration. Mechanistically, ELISA revealed the multi-pathway activity of CFE, promoting fibrinolysis through reductions in plasminogen, fibrinogen, and D-dimer; inhibiting platelet activation via downregulation of prostaglandin-endoperoxide synthase (PTGS), thromboxane A2 (TXA2), P-selectin, and von Willebrand factor (vWF); and modulating coagulation cascades through elevated protein C and tissue factor pathway inhibitor (TFPI) with concurrent suppression of coagulation factor VII (FVII).
Conclusions These results indicate that the fibrinolytic enzyme CFE, derived from Coprinus comatus, exerts potent antithrombotic effects, supporting its potential as a basis for fungal-derived natural antithrombotic functional food ingredients.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping