PUBLICATION
Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA
- Authors
- Jiang, Y., Guo, L., He, H., Chen, H., Chen, T., Liu, Y., Zhao, W.
- ID
- ZDB-PUB-250705-8
- Date
- 2025
- Source
- Frontiers in bioscience (Landmark edition) 30: 3958739587 (Journal)
- Registered Authors
- Keywords
- Lactobacillus reuteri, gamma-aminobutyric acid, sleep disorders, zebrafish
- MeSH Terms
-
- Probiotics*/pharmacology
- Animals
- Receptors, GABA/genetics
- Receptors, GABA/metabolism
- Pentylenetetrazole/toxicity
- Zebrafish
- Disease Models, Animal
- Gastrointestinal Microbiome
- Sleep Wake Disorders*/chemically induced
- Sleep Wake Disorders*/metabolism
- Sleep Wake Disorders*/therapy
- Limosilactobacillus reuteri*/metabolism
- Limosilactobacillus reuteri*/physiology
- gamma-Aminobutyric Acid*/metabolism
- Receptors, Melatonin/genetics
- Receptors, Melatonin/metabolism
- PubMed
- 40613302 Full text @ Front Biosci (Landmark Ed)
Citation
Jiang, Y., Guo, L., He, H., Chen, H., Chen, T., Liu, Y., Zhao, W. (2025) Lactobacillus reuteri E9 Regulates Sleep Disorders Through Its Metabolite GABA. Frontiers in bioscience (Landmark edition). 30:3958739587.
Abstract
Background Insomnia, the most prevalent sleep disorder, is clinically defined as difficulty initiating or maintaining sleep. Although many medications are effective for insomnia treatment, they carry risks of drug dependence and abuse. The microbiota-gut-brain axis (MGBA) facilitates bidirectional signaling between the gastrointestinal tract and the central nervous system via gut microbes. Probiotics that provide mental and behavioral benefits through MGBA (psychobiotics) offer broad therapeutic potential.
Methods A non-toxic, drug-resistant strain of Lactobacillus reuteri E9 was isolated and characterized. Its effects were evaluated in a pentylenetetrazol (PTZ)-induced zebrafish model of sleep disorder. Neurotransmitter levels (glycine, serine, taurine, γ-aminobutyric acid (GABA)) and gene expression of GABA/melatonin receptors were analyzed.
Results E9 significantly upregulated inhibitory neurotransmitters, including GABA, taurine, glycine, and serine (p < 0.05). In PTZ-induced zebrafish, E9 exerted sedative effects by reducing seizures and hyperactivity. Concurrently, E9 upregulated the expression of GABA receptor genes and melatonin receptor (Mtnr1aa) genes in zebrafish neural tissue.
Conclusions Lactobacillus reuteri E9 demonstrates potential as a psychobiotic for sleep disorder management by modulating key inhibitory neurotransmitters and sleep-related receptor expression via the MGBA pathway, offering a non-pharmacological alternative to conventional treatments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping