PUBLICATION
Zebrafish cdh23 Affects Rod Cell Phototransduction Through Regulating Ca2+ Transport and MAPK Signaling Pathway
- Authors
- Zheng, X., Xie, B., Chen, D., Jiang, J., Zeng, T., Xiong, L., Shi, Q., Xie, H., Cai, Y., Liang, J., Chen, S., Qu, X., Xie, H.
- ID
- ZDB-PUB-250528-26
- Date
- 2025
- Source
- International Journal of Molecular Sciences 26: (Journal)
- Registered Authors
- Xie, Binling, Xie, Huaping, Xiong, Lei, Zeng, Ting
- Keywords
- CDH23, Ca2+, MAPK, apoptosis, gene knockout, photoreceptor degeneration
- MeSH Terms
-
- Retinal Rod Photoreceptor Cells*/metabolism
- MAP Kinase Signaling System*
- Light Signal Transduction*
- Apoptosis
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Calcium*/metabolism
- Animals
- Cadherins*/genetics
- Cadherins*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 40429749 Full text @ Int. J. Mol. Sci.
Citation
Zheng, X., Xie, B., Chen, D., Jiang, J., Zeng, T., Xiong, L., Shi, Q., Xie, H., Cai, Y., Liang, J., Chen, S., Qu, X., Xie, H. (2025) Zebrafish cdh23 Affects Rod Cell Phototransduction Through Regulating Ca2+ Transport and MAPK Signaling Pathway. International Journal of Molecular Sciences. 26:.
Abstract
Mutations in the pathogenic gene CDH23 are known to cause Usher syndrome, affecting both auditory and visual functions. Our previous results provided valuable insights into the mechanisms underlying congenital hearing loss associated with CDH23 mutations. However, the molecular mechanisms and signaling pathways that influence vision remain largely unknown. In this study, transcriptional sequencing and bioinformatics analysis were conducted to compare gene expression between the control and cdh23-/-. Additionally, RT-qPCR experiments were performed to further validate the bioinformatics analysis results. The comparative transcriptomic analysis identified differentially expressed genes associated with photoreceptor degeneration and the mitogen-activated protein kinase (MAPK) signaling pathway. Embryos were subjected to hematoxylin and eosin (H&E) staining to assess their histological changes. The results showed that the cdh23-/- retina was morphologically indistinguishable from the control. Apoptosis was assessed using TUNEL staining, which revealed an increase in total cell death in the cdh23-/- retina. Our results revealed that the cell death was induced by Ca2+ and MAPK signaling interactions following photoreceptor degeneration. This study provides insights into the mechanisms underlying the role of cdh23 in vision.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping