PUBLICATION
Specific mitotic events drive left-right organizer development
- Authors
- Wu, Y., Lan, Y., Ononiwu, F., Poole, A., Rasmussen, K., Silva, J.D., Shamil, A.W., Jao, L.E., Hehnly, H.
- ID
- ZDB-PUB-250415-13
- Date
- 2025
- Source
- Development (Cambridge, England) : (Journal)
- Registered Authors
- Hehnly, Heidi, Jao, Li-En
- Keywords
- Cell Patterning, Cilia, Cytokinesis, Left-Right Organizer, Lumen Formation, Microtubules, Mitosis, Rosette
- MeSH Terms
-
- Zebrafish*/embryology
- Zebrafish*/genetics
- Mitosis*/physiology
- Body Patterning*/genetics
- Body Patterning*/physiology
- Antigens/genetics
- Antigens/metabolism
- Embryo, Nonmammalian/cytology
- Animals
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Microtubules/metabolism
- Spindle Apparatus/metabolism
- PubMed
- 40230260 Full text @ Development
Citation
Wu, Y., Lan, Y., Ononiwu, F., Poole, A., Rasmussen, K., Silva, J.D., Shamil, A.W., Jao, L.E., Hehnly, H. (2025) Specific mitotic events drive left-right organizer development. Development (Cambridge, England). :.
Abstract
Cell proliferation is crucial for tissue development. Here, we investigate its role in the Left-Right Organizer (LRO), which establishes the left-right (LR) axis. In zebrafish, we mapped mitotic events in Kupffer's Vesicle (KV) and identified an anteriorly enriched, FGF-dependent mitotic pattern. Laser ablation of mitotic cells and pericentrin-null mutants, both reducing mitotic events, resulted in smaller lumens, confirming that cell division is essential for KV development. Pericentrin-null mutants also exhibited defects in leftward cardiac jogging, indicative of KV dysfunction. Using a KV-specific fluorescent microtubule marker, we found that the KV rosette is a transient, centrally organized cluster interconnected by cytokinetic bridges and containing microtubule bundles. This structure emerges after the first four divisions and precedes lumen formation. Mitotic events during KV rounding coincide with rosette formation, spindle rotation, and cell extrusion, likely driven by increased packing. Eliminating the first four mitotic events disrupted rosette formation and prevented normal KV rounding. These findings demonstrate that mitotic events are critical for KV development, with cell division timing shaping KV architecture and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping