PUBLICATION
Age-dependent glial heterogeneity and traumatic injury responses in a vertebrate brain structure
- Authors
- Qin, H., Yu, S., Han, R., He, J.
- ID
- ZDB-PUB-250409-5
- Date
- 2025
- Source
- Cell Reports 44: 115508115508 (Journal)
- Registered Authors
- He, Jie, Qin, Huiwen, Yu, Shuguang
- Keywords
- CP: Cell biology, CP: Neuroscience, age dependent, glial dynamics, microglia, oligodendrocyte-lineage cells, post-embryonic developmental stage, radial astrocytes, traumatic brain injury, zebrafish optic tectum
- MeSH Terms
-
- Transcriptome
- Zebrafish
- Aging*/pathology
- Age Factors
- Brain*/metabolism
- Brain*/pathology
- Microglia/metabolism
- Microglia/pathology
- Animals
- Astrocytes/metabolism
- Brain Injuries, Traumatic*/genetics
- Brain Injuries, Traumatic*/metabolism
- Brain Injuries, Traumatic*/pathology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Neuroglia*/metabolism
- Neuroglia*/pathology
- Chemokine CXCL12/metabolism
- PubMed
- 40198221 Full text @ Cell Rep.
Citation
Qin, H., Yu, S., Han, R., He, J. (2025) Age-dependent glial heterogeneity and traumatic injury responses in a vertebrate brain structure. Cell Reports. 44:115508115508.
Abstract
The progression of traumatic brain injury (TBI) pathology is significantly influenced by age and involves a complex interplay of glial cells. However, the influence of age on the glial dynamics and their TBI responses remains mostly unexplored. Here, we obtain a comprehensive single-cell transcriptome atlas of three major glial types under the physiological and TBI conditions across four post-embryonic life stages in the zebrafish midbrain optic tectum. We identify a library of glial subtypes and states with specific age-dependent patterns that respond distinctly to TBI. Combining the glial interactome analysis and CRISPR-Cas9-mediated gene disruption, we reveal the essential roles of dla-notch3 and cxcl12a-cxcr4b interactions in the early-larval-stage-specific unresponsiveness of radial astrocytes to TBI and the TBI-induced age-independent recruitment of microglia to injury sites, respectively. Overall, our findings provide the molecular and cellular framework of TBI-induced age-related glial dynamics in vertebrate brains.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping