PUBLICATION
Amyloid-β Dysregulates Oligodendroglial Lineage Cell Dynamics and Myelination via PKC in the Zebrafish Spinal Cord
- Authors
- Balantzategi, U., Gaminde-Blasco, A., Kearns, C.A., Bayón-Cordero, L., Sánchez-Gómez, M.V., Zugaza, J.L., Appel, B., Alberdi, E.
- ID
- ZDB-PUB-250317-4
- Date
- 2025
- Source
- Glia : (Journal)
- Registered Authors
- Appel, Bruce, Kearns, Christina
- Keywords
- Alzheimer's disease, PKC, amyloid‐β, myelin, oligodendrocytes, spinal cord, zebrafish
- MeSH Terms
-
- Protein Kinase C*/metabolism
- Spinal Cord*/drug effects
- Spinal Cord*/metabolism
- Spinal Cord*/pathology
- Cell Differentiation/drug effects
- Animals, Genetically Modified
- Myelin Basic Protein/genetics
- Myelin Basic Protein/metabolism
- Amyloid beta-Peptides*/toxicity
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Myelin Sheath*/drug effects
- Myelin Sheath*/metabolism
- Cell Lineage*/drug effects
- Cell Lineage*/physiology
- Larva
- SOXE Transcription Factors/metabolism
- Zebrafish
- Oligodendrocyte Transcription Factor 2/genetics
- Oligodendrocyte Transcription Factor 2/metabolism
- Animals
- Oligodendroglia*/drug effects
- Oligodendroglia*/metabolism
- PubMed
- 40087862 Full text @ Glia
Citation
Balantzategi, U., Gaminde-Blasco, A., Kearns, C.A., Bayón-Cordero, L., Sánchez-Gómez, M.V., Zugaza, J.L., Appel, B., Alberdi, E. (2025) Amyloid-β Dysregulates Oligodendroglial Lineage Cell Dynamics and Myelination via PKC in the Zebrafish Spinal Cord. Glia. :.
Abstract
Soluble forms of amyloid-β (Aβ) peptide have been proposed as candidates to induce oligodendrocyte (OL) and myelin dysfunctions in the early stages of Alzheimer's disease (AD) pathology. Nevertheless, little is known about how Aβ affects OL differentiation and myelination in vivo, and the underlying molecular mechanisms. In this study, we explored the effects of a brain intraventricular injection of Aβ on OLs and myelin in the developing spinal cord of zebrafish larvae. Using quantitative fluorescent in situ RNA hybridization assays, we demonstrated that Aβ altered myrf and mbp mRNA levels and the regional distribution of mbp during larval development, suggesting an early differentiation of OLs. Through live imaging of Tg(myrf:mScarlet) and Tg(mbpa:tagRFP) zebrafish lines, both crossed with Tg(olig2:EGFP), we found that Aβ increased the number of myrf+ and mbp+ OLs in the dorsal spinal cord at 72 hpf and 5 dpf, respectively, without affecting total cell numbers. Furthermore, Aβ also increased the number of Sox10+cells, myelin sheaths per OL, and the number of myelinated axons in the dorsal spinal cord at 8 dpf compared to vehicle-injected control animals. Interestingly, the treatment of Aβ-injected zebrafish with the pan-PKC inhibitor Gö6983 restored the aforementioned alterations in OLs and myelin to control levels. Altogether, not only do we demonstrate that Aβ induces a precocious oligodendroglial differentiation leading to dysregulated myelination, but we also identified PKC as a key player in Aβ-induced pathology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping