PUBLICATION
Evaluation of the Anxiolytic and Anticonvulsant Effect of Marlierea eugeniopsoides Essential Oils and its Majority Constituents in Adult Zebrafish
- Authors
- Lima Mendes, J., Everson da Silva, L., do Amaral, W., Confortin, C., Kueirislene Amâncio Ferreira, M., Machado Marinho, M., Rogênio da Silva Mendes, F., Silva Marinho, E., Carneiro Romão, I., Paula Magalhães, E., Pessoa Bezerra de Menezes, R.P., Silva Alencar de Menezes, J.E., Dos Santos, H.S.
- ID
- ZDB-PUB-250307-10
- Date
- 2025
- Source
- Chemistry & biodiversity : e202403357e202403357 (Journal)
- Registered Authors
- Keywords
- Zebrafish, Anxiety, Convulsion, Marlierea eugeniopsoides
- MeSH Terms
-
- Dose-Response Relationship, Drug
- Molecular Structure
- Animals
- Oils, Volatile*/chemistry
- Oils, Volatile*/isolation & purification
- Oils, Volatile*/pharmacology
- Structure-Activity Relationship
- Molecular Docking Simulation
- Anticonvulsants*/chemistry
- Anticonvulsants*/isolation & purification
- Anticonvulsants*/pharmacology
- Pentylenetetrazole
- Seizures/chemically induced
- Seizures/drug therapy
- Behavior, Animal/drug effects
- Anti-Anxiety Agents*/chemistry
- Anti-Anxiety Agents*/isolation & purification
- Anti-Anxiety Agents*/pharmacology
- Zebrafish
- PubMed
- 40044596 Full text @ Chem. Biodivers.
Citation
Lima Mendes, J., Everson da Silva, L., do Amaral, W., Confortin, C., Kueirislene Amâncio Ferreira, M., Machado Marinho, M., Rogênio da Silva Mendes, F., Silva Marinho, E., Carneiro Romão, I., Paula Magalhães, E., Pessoa Bezerra de Menezes, R.P., Silva Alencar de Menezes, J.E., Dos Santos, H.S. (2025) Evaluation of the Anxiolytic and Anticonvulsant Effect of Marlierea eugeniopsoides Essential Oils and its Majority Constituents in Adult Zebrafish. Chemistry & biodiversity. :e202403357e202403357.
Abstract
Benzodiazepines are medications used to treat anxiety and epilepsy disorders. However, because of the existing adverse effects, new and safer medications are being sought. Therefore, this study aims to evaluate the chemical composition and the anxiolytic and anticonvulsant actions of the essential oil of Marlierea eugeniopsoides and its majority constituents α-Pinene and β-Pinene. The toxicity was analyzed, and the anxiolytic behavior of fish treated with the oil was assessed in light/dark tests and open field tests (n=6 animals/group). In the test to evaluate the anticonvulsant effect of the sample, Pentilenetetrazole (PTZ) was used to induce seizures through immersion. Subsequently, the mechanism of action was investigated with the γ-aminobutyric acid (GABA) antagonist, and molecular docking and ADMET study was conducted. The study revealed that Marlierea E. oil (spring and summer) was non-toxic and influenced zebrafish locomotion at all doses. Spring oil had anxiolytic effects at all doses, while summer oil showed this only at the highest dose. Both oils affected GABA, with spring oil displaying anticonvulsant properties, unlike summer oil, suggesting seasonal influence on oil behavior. Molecular docking indicated that inhibitors and ligands interacted beneficially, with α-pinene demonstrating superior inhibition and cellular permeability.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping