PUBLICATION
Soy Protein-Cultured Mesenchymal Stem Cell-Secreted Extracellular Vesicles Target the Neurovascular Unit: Insights from a Zebrafish Brain Injury Model
- Authors
- Lin, T.I., Hsieh, P.Y., Lin, H.J., Chiang, C.K., Sheu, J.J., Chang, W.T., Liau, I., Hsu, H.Y.
- ID
- ZDB-PUB-250226-16
- Date
- 2025
- Source
- ACS biomaterials science & engineering : (Journal)
- Registered Authors
- Keywords
- extracellular vesicles, neurovascular unit, soy protein isolates, zebrafish model
- MeSH Terms
-
- Cells, Cultured
- Zebrafish*
- Disease Models, Animal*
- Soybean Proteins*/pharmacology
- Extracellular Vesicles*/metabolism
- Extracellular Vesicles*/transplantation
- Brain Injuries*/metabolism
- Brain Injuries*/pathology
- Brain Injuries*/therapy
- Humans
- Mesenchymal Stem Cells*/metabolism
- Animals
- Blood-Brain Barrier*/metabolism
- Blood-Brain Barrier*/pathology
- PubMed
- 40000145 Full text @ ACS Biomater Sci Eng
Citation
Lin, T.I., Hsieh, P.Y., Lin, H.J., Chiang, C.K., Sheu, J.J., Chang, W.T., Liau, I., Hsu, H.Y. (2025) Soy Protein-Cultured Mesenchymal Stem Cell-Secreted Extracellular Vesicles Target the Neurovascular Unit: Insights from a Zebrafish Brain Injury Model. ACS biomaterials science & engineering. :.
Abstract
Cerebral vascular disorders often accompany hypoxia-induced brain injury. In this study, we develop a zebrafish model of hypoxia-induced cerebral vascular injury to replicate the associated phenotypic changes, including cerebrovascular damage, neuronal apoptosis, and neurological dysfunction. We then explored the therapeutic potential of extracellular vesicles derived from Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) cultured on soy protein-coated surfaces. These vesicles demonstrated superior recovery efficacy, especially in restoring the blood-brain barrier integrity and improving neurological function. Our findings suggest that these potent therapeutic extracellular vesicles, easily produced from WJ-MSCs cultured in the presence of soy proteins, may mitigate hypoxia-induced brain injury by decreasing the severity of vascular disorder caused by oxidative stress. Protein-protein interactome analysis further suggests that multiple signaling pathways are likely involved in restoring normal neurovascular unit function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping