PUBLICATION
Zbtb48 is a regulator of Mtfp1 expression in zebrafish
- Authors
- Goh, S.Y.C., Fradera-Sola, A., Wittkopp, N., ?erifo?lu, N., Godinho Ferreira, M., Ketting, R.F., Butter, F.
- ID
- ZDB-PUB-250223-14
- Date
- 2025
- Source
- Communications biology 8: 277277 (Journal)
- Registered Authors
- Ketting, René, Wittkopp, Nadine
- Keywords
- none
- MeSH Terms
-
- Zebrafish*/genetics
- Male
- Animals
- CRISPR-Cas Systems
- Gene Expression Regulation
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Female
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Gene Expression Regulation, Developmental
- PubMed
- 39987415 Full text @ Commun Biol
Citation
Goh, S.Y.C., Fradera-Sola, A., Wittkopp, N., ?erifo?lu, N., Godinho Ferreira, M., Ketting, R.F., Butter, F. (2025) Zbtb48 is a regulator of Mtfp1 expression in zebrafish. Communications biology. 8:277277.
Abstract
ZBTB48 (also known as TZAP) is a transcription factor that has previously been reported to bind to telomeres and act as a negative regulator of telomere length in human cell lines. To explore whether transcription factor activity and telomere length regulation are conserved at the organismal level in vertebrates, we generate a zbtb48-/- zebrafish line via CRISPR‒Cas genome editing. The zbtb48-/- mutants display no obvious physical or behavioral abnormalities in the first two generations. We find no statistically significant changes in telomere length in first-generation adults. However, for the gene regulatory aspect of Zbtb48, similar to that in human cancer cell lines, we observe downregulation of mtfp1 at both the mRNA and protein levels in the zbtb48-/- mutants. This suggests that mtfp1 is an evolutionarily conserved regulatory target of Zbtb48. Further investigation of the spatiotemporal expression of zbtb48 in previously published zebrafish data reveals low transcript expression in diverse tissues, except in germline stem cells and gametocytes of the gonads. Notably, Mtfp1 protein downregulation is detected in the ovaries of 40 dpf zbtb48-/- mutants and in the testes of both 40 dpf and 10.5-month-old zbtb48-/- mutants.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping