PUBLICATION
Transgenerational toxicity assessment of [C8mim]Br: Focus on early development, antioxidant defense, and transcriptome profiles in zebrafish
- Authors
- Zhao, F., Shen, Y., Ma, Z., Tian, H., Duan, B., Xiao, Y., Liu, C., Shi, X., Chen, D., Wei, W., Jiang, R., Wei, P.
- ID
- ZDB-PUB-250216-1
- Date
- 2025
- Source
- Ecotoxicology and environmental safety 291: 117884117884 (Journal)
- Registered Authors
- Keywords
- Ionic liquid, Offspring toxicity, Oxidative stress, Transcriptomic sequencing, Zebrafish
- MeSH Terms
-
- Water Pollutants, Chemical*/toxicity
- Transcriptome*/drug effects
- Embryonic Development/drug effects
- Ionic Liquids/toxicity
- Antioxidants*/metabolism
- Animals
- Larva/drug effects
- Oxidative Stress/drug effects
- Female
- Male
- Embryo, Nonmammalian/drug effects
- Zebrafish*
- Imidazoles/toxicity
- PubMed
- 39951882 Full text @ Ecotoxicol. Environ. Saf.
Citation
Zhao, F., Shen, Y., Ma, Z., Tian, H., Duan, B., Xiao, Y., Liu, C., Shi, X., Chen, D., Wei, W., Jiang, R., Wei, P. (2025) Transgenerational toxicity assessment of [C8mim]Br: Focus on early development, antioxidant defense, and transcriptome profiles in zebrafish. Ecotoxicology and environmental safety. 291:117884117884.
Abstract
1-Methyl-3-octylimidazolium bromide ([C8mim]Br), one of the most widely used and studied ionic liquids, is a contaminant of emerging concern. Acute exposure to high doses of [C8mim]Br has been shown to induce a variety of toxicity effects in exposed animals. However, the detrimental effects of chronic parental exposure to low doses of [C8mim]Br on unexposed offspring and the underlying mechanisms remain largely unknown. To this end, spawning-capable female and male zebrafish (F0 generation) were separately exposed to 25, 250, and 2500 μg/L of [C8mim]Br for eight weeks and were then mated to spawn. The resulting eggs (F1 generation) were collected and cultured in [C8mim]Br-free media for 96 h. We found that the early growth and development of F1 embryo-larvae, which were not directly exposed to [C8mim]Br, were significantly inhibited. This was evidenced by delayed hatching, increased mortality, reduced body weight, slowed heartbeat, poor motility (decreased spontaneous tail-coiling movements and diminished escape responses to touch stimuli and water swirling), and uninflated swim bladders. Furthermore, fluorescent probe labeling and biochemical analyses revealed an accumulation of reactive oxygen species and impairment of the antioxidant defense system in F1 larvae from [C8mim]Br-exposed F0 parents, indicating the induction of oxidative stress. Finally, transcriptomic sequencing demonstrated that the differentially expressed genes in F1 larvae were primarily involved in muscle development and contraction performance, offering mechanistic insights into the poor motility and associated developmental defects observed in F1 embryo-larvae. Overall, this transgenerational toxicity assessment underscores the adverse outcomes of parental [C8mim]Br exposure on unexposed offspring, providing a crucial aspect of the ecological risks of [C8mim]Br.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping