PUBLICATION
Protocol for quantifying xenografted human cancer cells in zebrafish larvae using Cellpose
- Authors
- Köpke, K., Lembke, C.S., Oosterhof, N., Dijkstra, E.S.C., Paridaen, J.T.M.L.
- ID
- ZDB-PUB-241216-2
- Date
- 2024
- Source
- STAR protocols 5: 103479103479 (Journal)
- Registered Authors
- Keywords
- Cancer, Computer sciences, Model Organisms
- MeSH Terms
-
- Glioblastoma/pathology
- Neoplasm Transplantation
- Humans
- Neoplastic Stem Cells/pathology
- Transplantation, Heterologous/methods
- Heterografts
- Algorithms
- Larva*/cytology
- Zebrafish*
- Animals
- PubMed
- 39656591 Full text @ STAR Protoc
Citation
Köpke, K., Lembke, C.S., Oosterhof, N., Dijkstra, E.S.C., Paridaen, J.T.M.L. (2024) Protocol for quantifying xenografted human cancer cells in zebrafish larvae using Cellpose. STAR protocols. 5:103479103479.
Abstract
Here, we present a protocol for quantifying xenografted human glioblastoma stem cells (GSCs) in zebrafish larvae. We first describe steps for orthotopic xenotransplantation of GSCs into the midbrain of zebrafish larvae, immunofluorescent labeling, and confocal imaging. We then detail procedures for GSC quantification using the Cellpose algorithm. This protocol provides a technique for the semi-automatic segmentation and quantification of human cancer cells in xenograft experiments. With this approach, cancer cell survival and proliferation can be determined in an unbiased manner.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping