PUBLICATION
cpt1b Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish
- Authors
- Cheng, X., Ju, J., Huang, W., Duan, Z., Han, Y.
- ID
- ZDB-PUB-241127-16
- Date
- 2024
- Source
- Journal of cardiovascular development and disease 11(11): (Journal)
- Registered Authors
- Han, Yanchao
- Keywords
- cardiomyocyte proliferation, cpt1b, glul, metabolism, zebrafish
- MeSH Terms
- none
- PubMed
- 39590187 Full text @ J Cardiovasc Dev Dis
Citation
Cheng, X., Ju, J., Huang, W., Duan, Z., Han, Y. (2024) cpt1b Regulates Cardiomyocyte Proliferation Through Modulation of Glutamine Synthetase in Zebrafish. Journal of cardiovascular development and disease. 11(11):.
Abstract
Carnitine palmitoyltransferase 1b (Cpt1b) is a crucial rate-limiting enzyme in fatty acid metabolism, but its role and mechanism in early cardiac development remains unclear. Here, we show that cpt1b regulates cardiomyocyte proliferation during zebrafish development. Knocking out entire cpt1b coding sequences leads to impaired cardiomyocyte proliferation, while cardiomyocyte-specific overexpression of cpt1b promotes cardiomyocyte proliferation. RNA sequencing analysis and pharmacological studies identified glutamine synthetase as a key downstream effector of cpt1b in regulating cardiomyocyte proliferation. Our study elucidates a novel mechanism whereby cpt1b promotes zebrafish cardiomyocyte proliferation through glutamine synthetase, which provides new perspectives on the significance of fatty acid metabolism in heart development and the interplay between fatty acid and amino acid metabolic pathways.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping