PUBLICATION

Integrated Studies on Male Reproductive Toxicity of Decabromodiphenyl Ethane in Zebrafish Spermatozoa Ex Vivo, Male Zebrafish in Vivo, and GC-1 Cells in Vitro

Authors
Yang, L., Zhang, Y., Hua, J., Song, G., Li, F., Zheng, N., Zhang, T., Xu, Z., Ren, X., Zhu, B., Han, Y., Guo, Y., Han, J., Zhou, B.
ID
ZDB-PUB-241122-3
Date
2024
Source
Environmental health perspectives   132: 117005117005 (Journal)
Registered Authors
Song, Guili, Zhou, BingSheng
Keywords
none
MeSH Terms
  • DNA Damage
  • Reproduction/drug effects
  • Male
  • Spermatozoa*/drug effects
  • Zebrafish*
  • Bromobenzenes/toxicity
  • Mice
  • Flame Retardants*/toxicity
  • Testis/drug effects
  • Animals
  • Sperm Motility/drug effects
PubMed
39570742 Full text @ Environ. Health Perspect.
Citation
Yang, L., Zhang, Y., Hua, J., Song, G., Li, F., Zheng, N., Zhang, T., Xu, Z., Ren, X., Zhu, B., Han, Y., Guo, Y., Han, J., Zhou, B. (2024) Integrated Studies on Male Reproductive Toxicity of Decabromodiphenyl Ethane in Zebrafish Spermatozoa Ex Vivo, Male Zebrafish in Vivo, and GC-1 Cells in Vitro. Environmental health perspectives. 132:117005117005.
Abstract
Background Legacy brominated flame retardants have been recognized as risky factors leading to declined sperm quality. The widespread utilization of decabromodiphenyl ethane (DBDPE) as a replacement for decabromodiphenyl ether has given rise to considerable concern over its potential risks to reproductive health.
Objectives The objectives were to quickly determine whether DBDPE affects sperm quality upon ex vivo exposure, to reveal the reproductive outcomes and underlying molecular mechanisms using an in vivo zebrafish model exposed to DBDPE, and to validate the potential impact on DNA damage and energy metabolism balance in vitro.
Methods Zebrafish spermatozoa were treated with DBDPE (0.01, 0.1, 1, 10μM) for 3 h, and the spermatozoa motility and fertilization ability with normal eggs were evaluated. Then adult male zebrafish were treated with DBDPE (0.1, 1, 10, and 100 nM) for 2 months, and their reproductive performance was examined. Four-dimensional label-free proteome and phosphoproteome were performed in zebrafish testes, and the findings were validated by multiple indicators. Finally, mouse spermatogonial GC-1 cells were treated with DBDPE (0.1, 1μM) for 72 h, and DNA damage was examined, as well as the energy production of glycolysis and oxidative phosphorylation.
Results Ex vivo exposure to DBDPE caused lower motility and fertilization rates of zebrafish spermatozoa. In vivo exposure to DBDPE caused lower sperm motility and abnormal spermatogenesis in male zebrafish testes. Integrated whole-proteome and phosphoproteome analysis revealed DNA damage responses and energy metabolic disorders in zebrafish testes. A dosage window characterized by higher mitochondrial membrane potential (MMP) in combination with unchanged reactive oxygen species and apoptosis rates was observed in both zebrafish testes and GC-1 cells.
Discussion This study suggests that in zebrafish, DBDPE exposure could impair sperm quality and spermatogenesis, and the underlying mechanism could be related to DNA damage and energy metabolic reprogramming in testicular germ cells. https://doi.org/10.1289/EHP14426.
Genes / Markers
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Phenotype
Mutations / Transgenics
Human Disease / Model
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Antibodies
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Mapping
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