PUBLICATION
Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells
- Authors
- Morales Fénero, C., Sacksteder, R.E., Diamos, A.G., Kimmey, J.M.
- ID
- ZDB-PUB-241114-80
- Date
- 2024
- Source
- Scientific Reports 14: 2780527805 (Journal)
- Registered Authors
- Morales Fénero, Camila
- Keywords
- none
- MeSH Terms
-
- Light
- Streptococcus pneumoniae*/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Hot Temperature
- Cryptochromes/genetics
- Cryptochromes/metabolism
- Circadian Clocks*/genetics
- Cell Line
- Gene Expression Regulation/radiation effects
- Period Circadian Proteins/genetics
- Period Circadian Proteins/metabolism
- Zebrafish*/microbiology
- Animals
- PubMed
- 39537820 Full text @ Sci. Rep.
Citation
Morales Fénero, C., Sacksteder, R.E., Diamos, A.G., Kimmey, J.M. (2024) Heat-inactivated Streptococcus pneumoniae augments circadian clock gene expression in zebrafish cells. Scientific Reports. 14:2780527805.
Abstract
The circadian clock is a cell-autonomous process that regulates daily internal rhythms by interacting with environmental signals. Reports across species show that infection can alter the expression of circadian genes; however, in teleosts, these effects are influenced by light exposure. Currently, no reports analyze the direct effects of bacterial exposure on the zebrafish clock. Using zebrafish Z3 cells, we demonstrate that exposure to heat-killed Streptococcus pneumoniae (HK-Spn) augments the expression of core repressive factors in a light- and time-dependent manner. In constant darkness, HK-Spn highly upregulated cry1a, per3, and per1b expression. In the presence of light, HK-Spn exposure rapidly and strongly upregulated per2 and cry1a, and this was proportionally increased with light intensity. The combinatorial effect of light and HK-Spn on per2 and cry1a was not duplicated with H2O2, a known byproduct of light exposure. However, the ROS inhibitor N-acetyl cysteine was sufficient to block HK-Spn augmentation of per2, cry1a, and per3. These findings demonstrate that exposure to an inactive bacteria influences the expression of zebrafish clock genes under different light conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping