PUBLICATION
Samd7 represses short-wavelength cone genes to preserve long-wavelength cone and rod photoreceptor identity
- Authors
- Volkov, L.I., Ogawa, Y., Somjee, R., Vedder, H.E., Powell, H.E., Poria, D., Meiselman, S., Kefalov, V.J., Corbo, J.C.
- ID
- ZDB-PUB-241114-33
- Date
- 2024
- Source
- Proceedings of the National Academy of Sciences of the United States of America 121: e2402121121e2402121121 (Journal)
- Registered Authors
- Corbo, Joseph, Ogawa, Yohey, Volkov, Leo
- Keywords
- development, photoreceptor, retina
- Datasets
- GEO:GSE250379, GEO:GSE249756, GEO:GSE249754, GEO:GSE249755, GEO:GSE249751
- MeSH Terms
-
- Mice, Knockout
- Animals
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Gene Expression Regulation
- Cell Differentiation/genetics
- Zebrafish*/genetics
- Retinal Cone Photoreceptor Cells*/metabolism
- Retinal Rod Photoreceptor Cells*/metabolism
- Repressor Proteins/genetics
- Repressor Proteins/metabolism
- Mice
- PubMed
- 39531499 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Volkov, L.I., Ogawa, Y., Somjee, R., Vedder, H.E., Powell, H.E., Poria, D., Meiselman, S., Kefalov, V.J., Corbo, J.C. (2024) Samd7 represses short-wavelength cone genes to preserve long-wavelength cone and rod photoreceptor identity. Proceedings of the National Academy of Sciences of the United States of America. 121:e2402121121e2402121121.
Abstract
The role of transcription factors in photoreceptor gene regulation is fairly well understood, but knowledge of the cell-type-specific function of transcriptional cofactors remains incomplete. Here, we show that the transcriptional corepressor samd7 promotes rod differentiation and represses short-wavelength cone genes in long-wavelength cones in zebrafish. In samd7-/- retinas, red cones are transformed into hybrid red/ultraviolet (UV) cones, green cones are absent, the number of blue cones is approximately doubled, and the number of rods is greatly reduced. We also find that mouse Samd7 represses S-opsin expression in dorsal M-cones-analogous to its role in repressing UV cone genes in zebrafish red cones. Thus, samd7 plays a key role in ensuring appropriate patterns of gene expression in rods and cone subtypes of both zebrafish and mice.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping