PUBLICATION
Pharmacological Inhibition of the Spliceosome SF3b Complex by Pladienolide-B Elicits Craniofacial Developmental Defects in Mouse and Zebrafish
- Authors
- Hoshino, Y., Liu, S., Furutera, T., Yamada, T., Koyabu, D., Nukada, Y., Miyazawa, M., Yoda, T., Ichimura, K., Iseki, S., Tasaki, J., Takechi, M.
- ID
- ZDB-PUB-241105-3
- Date
- 2024
- Source
- Birth defects research 116: e2404e2404 (Journal)
- Registered Authors
- Keywords
- RNA splicing, SF3b complex, neural crest cell, neural tube defect, pladienolide?B, spliceosomopathy
- MeSH Terms
-
- Neural Tube Defects/genetics
- Disease Models, Animal
- Phenotype
- Macrolides
- Humans
- Apoptosis/drug effects
- Mice
- Epoxy Compounds/pharmacology
- Craniofacial Abnormalities*/genetics
- Neural Crest*/drug effects
- Neural Crest*/metabolism
- Spliceosomes*/drug effects
- Spliceosomes*/metabolism
- Animals
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- RNA Splicing Factors*/genetics
- RNA Splicing Factors*/metabolism
- Mice, Knockout
- Cell Proliferation/drug effects
- Zebrafish*/embryology
- PubMed
- 39494782 Full text @ Birth Defects Res
Citation
Hoshino, Y., Liu, S., Furutera, T., Yamada, T., Koyabu, D., Nukada, Y., Miyazawa, M., Yoda, T., Ichimura, K., Iseki, S., Tasaki, J., Takechi, M. (2024) Pharmacological Inhibition of the Spliceosome SF3b Complex by Pladienolide-B Elicits Craniofacial Developmental Defects in Mouse and Zebrafish. Birth defects research. 116:e2404e2404.
Abstract
Background Mutations in genes encoding spliceosome components result in craniofacial structural defects in humans, referred to as spliceosomopathies. The SF3b complex is a crucial unit of the spliceosome, but model organisms generated through genetic modification of the complex do not perfectly mimic the phenotype of spliceosomopathies. Since the phenotypes are suggested to be determined by the extent of spliceosome dysfunction, an alternative experimental system that can seamlessly control SF3b function is needed.
Methods To establish another experimental system for model organisms elucidating relationship between spliceosome function and human diseases, we administered Pladienolide-B (PB), a SF3b complex inhibitor, to mouse and zebrafish embryos and assessed resulting phenotypes.
Results PB-treated mouse embryos exhibited neural tube defect and exencephaly, accompanied by apoptosis and reduced cell proliferation in the neural tube, but normal structure in the midface and jaw. PB administration to heterozygous knockout mice of Sf3b4, a gene coding for a SF3b component, influenced the formation of cranial neural crest cells (CNCCs). Despite challenges in continuous PB administration and a high death rate in mice, PB was stably administered to zebrafish embryos, resulting in prolonged survival. Brain, cranial nerve, retina, midface, and jaw development were affected, mimicking spliceosomopathy phenotypes. Additionally, alterations in cell proliferation, cell death, and migration of CNCCs were detected.
Conclusions We demonstrated that zebrafish treated with PB exhibited phenotypes similar to those observed in human spliceosomopathies. This experimental system may serve as a valuable research tool for understanding spliceosome function and human diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping