PUBLICATION

Role of Y box-binding protein 1 (Ybx1) in zebrafish folliculogenesis: Promoting follicle cell proliferation via suppression of p21 (cdkn1a)

Authors
Zhu, B., Zhang, Z., Pardeshi, L., Chen, Y., Ge, W.
ID
ZDB-PUB-241030-1
Date
2024
Source
Development (Cambridge, England)   151(21): (Journal)
Registered Authors
Ge, Wei, Zhang, Zhiwei, Zhu, Bo
Keywords
CRISPR/Cas9, Gametogenesis, Ovarian folliculogenesis, Y box-binding protein, Zebrafish, p21 cdkn1a
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Animals
  • Cell Proliferation*/genetics
  • Y-Box-Binding Protein 1*/genetics
  • Y-Box-Binding Protein 1*/metabolism
  • Cyclin-Dependent Kinase Inhibitor p21*/genetics
  • Cyclin-Dependent Kinase Inhibitor p21*/metabolism
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
  • Female
  • Ovarian Follicle*/metabolism
  • Zebrafish*/embryology
  • Zebrafish*/metabolism
PubMed
39470059 Full text @ Development
Abstract
Y box-binding protein 1 (YB-1; Ybx1/ybx1) regulates gene expression through DNA/RNA-binding. In zebrafish, Ybx1 is highly abundant in primary growth (PG) follicles in the ovary, but decreases precipitously as the follicles enter the secondary growth (SG). To understand Ybx1 function in folliculogenesis, we created an ybx1 mutant using TALEN and observed disrupted folliculogenesis during the previtellogenic (PV) to early vitellogenic (EV) transition of SG, resulting in underdeveloped ovaries and infertility. Expression and Western blot analyses revealed differential gene expression between ybx1-/- and control ovaries, with significantly increased expression of cdkn1a (p21), a cell cycle inhibitor, in ybx1-/- follicles. While cdkn1a knockout via CRISPR/Cas9 was embryonically lethal, the heterozygote (cdkn1a+/-) displayed advanced follicle activation and maturation, contrasting with the ybx1-/- phenotype. Partial loss of p21 alleviated the ybx1-/-phenotype, restoring folliculogenesis with normal PG-PV and PV-EV transitions in ybx1-/-;cdkn1a+/- mutant. While ybx1-/- mutant follicle cells displayed poor proliferation in vivo and in vitro, the cells from the ybx1-/-;p21+/- follicles resumed normal proliferation. In conclusion, Ybx1 is crucial for early folliculogenesis in zebrafish, potentially by repressing cdkn1a expression, either directly or indirectly.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping