PUBLICATION
Zonated Wnt/β-catenin signal-activated cardiomyocytes at the atrioventricular canal promote coronary vessel formation in zebrafish
- Authors
- Chiba, A., Yamamoto, T., Fukui, H., Fukumoto, M., Shirai, M., Nakajima, H., Mochizuki, N.
- ID
- ZDB-PUB-241013-8
- Date
- 2024
- Source
- Developmental Cell 60(1): 21-29.e8 (Journal)
- Registered Authors
- Fukui, Hajime, Mochizuki, Naoki, Nakajima, Hiroyuki
- Keywords
- Wnt/β-catenin signaling, angiogenesis, arterialization, atrioventricular canal, coronary vessels, heartbeat, zonation
- Datasets
- GEO:GSE261604, GEO:GSE223462, GEO:GSE223461, GEO:GSE223460
- MeSH Terms
-
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Endothelial Cells/cytology
- Endothelial Cells/metabolism
- Coronary Vessels*/cytology
- Coronary Vessels*/embryology
- Coronary Vessels*/metabolism
- Wnt Signaling Pathway*
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Myocytes, Cardiac*/cytology
- Myocytes, Cardiac*/metabolism
- Gene Expression Regulation, Developmental
- Heart Atria/cytology
- Heart Atria/embryology
- Heart Atria/metabolism
- Animals
- beta Catenin*/metabolism
- PubMed
- 39395410 Full text @ Dev. Cell
Citation
Chiba, A., Yamamoto, T., Fukui, H., Fukumoto, M., Shirai, M., Nakajima, H., Mochizuki, N. (2024) Zonated Wnt/β-catenin signal-activated cardiomyocytes at the atrioventricular canal promote coronary vessel formation in zebrafish. Developmental Cell. 60(1):21-29.e8.
Abstract
Cells functioning at a specific zone by clustering according to gene expression are recognized as zonated cells. Here, we demonstrate anatomical and functional zones in the zebrafish heart. The cardiomyocytes (CMs) at the atrioventricular canal between the atrium and ventricle could be grouped into three zones according to the localization of signal-activated CMs: Wnt/β-catenin signal+, Bmp signal+, and Tbx2b+ zones. Endocardial endothelial cells (ECs) changed their characteristics, penetrated the Wnt/β-catenin signal+ CM zone, and became coronary ECs covering the heart. Coronary vessel length was reduced when the Wnt/β-catenin signal+ CMs were depleted. Collectively, we demonstrate the importance of anatomical and functional zonation of CMs in the zebrafish heart.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping