PUBLICATION
The functional roles of zebrafish HoxA- and HoxD-related clusters in the pectoral fin development
- Authors
- Ishizaka, M., Maeno, A., Nakazawa, H., Fujii, R., Oikawa, S., Tani, T., Kanno, H., Koita, R., Kawamura, A.
- ID
- ZDB-PUB-241011-12
- Date
- 2024
- Source
- Scientific Reports 14: 2360223602 (Journal)
- Registered Authors
- Kawamura, Akinori
- Keywords
- Hox genes, Pectoral fin, X-ray CT scan, Zebrafish
- MeSH Terms
-
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Gene Expression Regulation, Developmental
- Animal Fins*/growth & development
- Animal Fins*/metabolism
- Animals
- Zebrafish*/genetics
- Homeodomain Proteins*/genetics
- Homeodomain Proteins*/metabolism
- Multigene Family*
- Mutation
- PubMed
- 39384796 Full text @ Sci. Rep.
Citation
Ishizaka, M., Maeno, A., Nakazawa, H., Fujii, R., Oikawa, S., Tani, T., Kanno, H., Koita, R., Kawamura, A. (2024) The functional roles of zebrafish HoxA- and HoxD-related clusters in the pectoral fin development. Scientific Reports. 14:2360223602.
Abstract
The paralogs 9-13 Hox genes in mouse HoxA and HoxD clusters are critical for limb development. When both HoxA and HoxD clusters are deleted in mice, significant limb truncation is observed compared to the phenotypes of single and compound mutants of Hox9-13 genes in these clusters. In zebrafish, mutations in hox13 genes in HoxA- and HoxD-related clusters result in abnormal morphology of pectoral fins, homologous to forelimbs. However, the effect of the simultaneous deletions of entire HoxA- and HoxD-related clusters on pectoral fin development remains unknown. Here, we generated mutants with several combinations of hoxaa, hoxab, and hoxda cluster deletions and analyzed the pectoral fin development. In hoxaa-/-;hoxab-/-;hoxda-/- larvae, the endoskeletal disc and the fin-fold are significantly shortened in developing pectoral fins. In addition, we show that this anomaly is due to defects in the pectoral fin growth after the fin bud formation. Furthermore, in the surviving adult mutants, micro-CT scanning reveals defects in the posterior portion of the pectoral fin which is thought to represent latent regions of the limb. Our results further support that the functional role of HoxA and HoxD clusters is conserved in the paired appendage formation in bony fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping