PUBLICATION
Staphylococcus aureus lipid factors modulate melanoma cell clustering and invasion
- Authors
- Giese, M.A., Ramakrishnan, G., Steenberge, L.H., Dovan, J.X., Sauer, J.D., Huttenlocher, A.
- ID
- ZDB-PUB-240918-9
- Date
- 2024
- Source
- Disease models & mechanisms 17(9): (Journal)
- Registered Authors
- Huttenlocher, Anna
- Keywords
- S. aureus, Lipids, Melanoma, Skin microbiome, Zebrafish
- MeSH Terms
-
- Cell Line, Tumor
- Lipids/chemistry
- Larva/microbiology
- Melanoma*/microbiology
- Melanoma*/pathology
- Cell Movement/drug effects
- Humans
- Animals
- Zebrafish*/microbiology
- Cell Aggregation/drug effects
- Staphylococcus aureus*
- Neoplasm Invasiveness*
- Zebrafish Proteins/metabolism
- PubMed
- 39284707 Full text @ Dis. Model. Mech.
Citation
Giese, M.A., Ramakrishnan, G., Steenberge, L.H., Dovan, J.X., Sauer, J.D., Huttenlocher, A. (2024) Staphylococcus aureus lipid factors modulate melanoma cell clustering and invasion. Disease models & mechanisms. 17(9):.
Abstract
The microbiome can influence cancer development and progression. However, less is known about the role of the skin microbiota in melanoma. Here, we took advantage of a zebrafish melanoma model to probe the effects of Staphylococcus aureus on melanoma invasion. We found that S. aureus produces factors that enhance melanoma invasion and dissemination in zebrafish larvae. We used a published in vitro 3D cluster formation assay that correlates increased clustering with tumor invasion. S. aureus supernatant increased clustering of melanoma cells and was abrogated by a Rho-Kinase inhibitor, implicating a role for Rho-GTPases. The melanoma clustering response was specific to S. aureus but not to other staphylococcal species, including S. epidermidis. Our findings suggest that S. aureus promotes melanoma clustering and invasion via lipids generated by the lipase Sal2 (officially known as GehB). Taken together, these findings suggest that specific bacterial products mediate melanoma invasive migration in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping