PUBLICATION
Genetic and Environmental Factors Co-Contributing to Behavioral Abnormalities in adnp/adnp2 Mutant Zebrafish
- Authors
- Wang, Y., Sun, X., Xiong, B., Duan, M., Sun, Y.
- ID
- ZDB-PUB-240915-19
- Date
- 2024
- Source
- International Journal of Molecular Sciences 25(17): (Journal)
- Registered Authors
- Sun, Xiaoyun
- Keywords
- adnp, adnp2, environmental endocrine disruptors, neurodevelopmental disorders, zebrafish
- MeSH Terms
-
- Animals
- Endocrine Disruptors/toxicity
- Disease Models, Animal
- Brain/drug effects
- Brain/metabolism
- Neurodevelopmental Disorders/chemically induced
- Neurodevelopmental Disorders/genetics
- Behavior, Animal/drug effects
- CRISPR-Cas Systems
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Nerve Tissue Proteins*/genetics
- Nerve Tissue Proteins*/metabolism
- Gene-Environment Interaction
- Autism Spectrum Disorder/genetics
- Zebrafish*/genetics
- Mutation*
- PubMed
- 39273418 Full text @ Int. J. Mol. Sci.
Citation
Wang, Y., Sun, X., Xiong, B., Duan, M., Sun, Y. (2024) Genetic and Environmental Factors Co-Contributing to Behavioral Abnormalities in adnp/adnp2 Mutant Zebrafish. International Journal of Molecular Sciences. 25(17):.
Abstract
Human mutations of ADNP and ADNP2 are known to be associated with neural developmental disorders (NDDs), including autism spectrum disorders (ASDs) and schizophrenia (SZ). However, the underlying mechanisms remain elusive. In this study, using CRISPR/Cas9 gene editing technology, we generated adnp and adnp2 mutant zebrafish models, which exhibited developmental delays, brain deficits, and core behavioral features of NDDs. RNA sequencing analysis of adnpa-/-; adnpb-/- and adnp2a-/-; adnp2b-/- larval brains revealed altered gene expression profiles affecting synaptic transmission, autophagy, apoptosis, microtubule dynamics, hormone signaling, and circadian rhythm regulation. Validation using whole-mount in situ hybridization (WISH) and real-time quantitative PCR (qRT-PCR) corroborated these findings, supporting the RNA-seq results. Additionally, loss of adnp and adnp2 resulted in significant downregulation of pan-neuronal HuC and neuronal fiber network α-Tubulin signals. Importantly, prolonged low-dose exposure to environmental endocrine disruptors (EEDs) aggravated behavioral abnormalities in adnp and adnp2 mutants. This comprehensive approach enhances our understanding of the complex interplay between genetic mutations and environmental factors in NDDs. Our findings provide novel insights and experimental foundations into the roles of adnp and adnp2 in neurodevelopment and behavioral regulation, offering a framework for future preclinical drug screening aimed at elucidating the pathogenesis of NDDs and related conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping