PUBLICATION
Induction of seizures and initiation of epileptogenesis by pilocarpine in zebrafish larvae
- Authors
- Gawel, K., Hulas-Stasiak, M., Marszalek-Grabska, M., Grenda, A., Siekierska, A., Kosheva, N., van der Ent, W., Esguerra, C.V., Krawczyk, P., Turski, W.A.
- ID
- ZDB-PUB-240822-3
- Date
- 2024
- Source
- Frontiers in molecular neuroscience 17: 14186061418606 (Journal)
- Registered Authors
- Esguerra, Camila V., Gawel, Kinga Aurelia, Marszalek-Grabska, Marta, Turski, Waldemar, van der Ent, Wietske
- Keywords
- epileptogenesis, local field potential recordings, locomotor activity, pilocarpine, seizure, zebrafish
- MeSH Terms
- none
- PubMed
- 39165716 Full text @ Front. Mol. Neurosci.
Citation
Gawel, K., Hulas-Stasiak, M., Marszalek-Grabska, M., Grenda, A., Siekierska, A., Kosheva, N., van der Ent, W., Esguerra, C.V., Krawczyk, P., Turski, W.A. (2024) Induction of seizures and initiation of epileptogenesis by pilocarpine in zebrafish larvae. Frontiers in molecular neuroscience. 17:14186061418606.
Abstract
Objective Preclinical models of seizures and epilepsy in rodents contributed substantially to the discovery of currently available antiseizure medications. These were also broadly used for investigation of processes of epileptogenesis. Nevertheless, rodent models pose some limitations, thus, new models using alternative species are in high demand. The aim of this study was to describe a new model of seizures/epilepsy induced by the cholinomimetic agent, pilocarpine (PILO), in larval zebrafish.
Methods Local field potential (LFP) recordings were conducted to analyze electroencephalographic discharges and correlate it with larval behavior. Hematoxylin and eosin (H&E) staining, as well as TUNEL staining were performed to analyze morphology and apoptosis, respectively. Real-time quantitative polymerase chain reaction (qRT-PCR) was undertaken for gene expression analysis.
Results Acute exposure to PILO, in a concentration-dependent manner, induces electroencephalographic discharges in larval zebrafish, which behaviorally manifest as decreased locomotion and moving time, but enhanced movement velocity. The PILO-induced seizure-like activity is behaviorally distinct from this induced by the application of chemoconvulsant pentylenetetrazole (PTZ). Zebrafish larvae previously exposed to PILO (2 h), after a washing out period, exhibit spontaneous, unprovoked discharges and apoptotic changes in their brains.
Significance Here, we comprehensively investigated a new model of PILO-induced seizures/epilepsy in larval zebrafish. We propose that this model may be used to study epileptogenesis and for antiseizure drug screening purposes.
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Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping