PUBLICATION
Cxcr4a regulates heart progenitor development and cardiac rhythm in zebrafish
- Authors
- Chen, B., Ran, Q., Chen, X., Deng, Z., Zhou, R., Zhang, Y., Liu, M., Li, B., Huang, S., Wang, P., Huang, S.
- ID
- ZDB-PUB-240807-9
- Date
- 2024
- Source
- Biochemistry and biophysics reports 39: 101782101782 (Journal)
- Registered Authors
- Huang, Sizhou
- Keywords
- Cardiac rhythm, Cxcr4a, Heart progenitor, RA signaling, Zebrafish
- MeSH Terms
- none
- PubMed
- 39108621 Full text @ Biochem Biophys Rep
Citation
Chen, B., Ran, Q., Chen, X., Deng, Z., Zhou, R., Zhang, Y., Liu, M., Li, B., Huang, S., Wang, P., Huang, S. (2024) Cxcr4a regulates heart progenitor development and cardiac rhythm in zebrafish. Biochemistry and biophysics reports. 39:101782101782.
Abstract
Cxcr4a is involved in multiple organ development including coronary vasculature formation and heart left-right (LR) patterning, whether it is involved in heart progenitor determination and cardiac rhythm regulation is not addressed. Here we showed that in cxcr4a mutants, from 2 days post fertilization (dpf) to 4dpf the embryos transiently displayed pericardial edema and increased cardiac rhythm. While from 5dpf, the heart phenotype disappeared. Detailed analysis demonstrated that, at 36hpf and 48hpf, even though there was no distinct difference in the heart size between cxcr4a mutants and controls, the expression of myl7 was decreased. Further data showed that, the heart progenitors were decreased at 18SS(Somite Stage). Mechanically, RNA-seq, RT-qPCR and in situ experiments showed that the retinoic acid (RA) signaling was upregulated, and the up-regulation of RA signaling may mediate the role of cxcr4a in regulating heart progenitor development. In addition, we also identified that low dose of RA treatment accelerated the cardiac rhythm, being similar to that in cxcr4a mutants. Decreasing RA signaling partially restored the rapid cardiac rhythm in cxcr4a mutants, implying the possibility that RA signaling partially mediates the role of cxcr4a in regulating cardiac rhythm. In conclusion, our study identified cxcr4a simultaneously regulates heart progenitor determination and cardiac rhythm.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping