PUBLICATION
Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae
- Authors
- Ariani, A., Khotimah, H., Sulistyarini, A., Daniaridevi, A.S.
- ID
- ZDB-PUB-240628-11
- Date
- 2024
- Source
- Open veterinary journal 14: 115411601154-1160 (Journal)
- Registered Authors
- Keywords
- Centella asiatica, Neuroplasticity, Neuroprotective, Neurotoxicity
- MeSH Terms
-
- Animals
- Brain-Derived Neurotrophic Factor*/genetics
- Brain-Derived Neurotrophic Factor*/metabolism
- Centella*/chemistry
- Fish Diseases/chemically induced
- Fish Diseases/drug therapy
- Hypoxia/drug therapy
- Hypoxia/veterinary
- Larva*/drug effects
- Larva*/growth & development
- Neuroprotective Agents/pharmacology
- Oxygen/metabolism
- Plant Extracts*/administration & dosage
- Plant Extracts*/pharmacology
- Triterpenes*/administration & dosage
- Triterpenes*/pharmacology
- Zebrafish*
- PubMed
- 38938421 Full text @ Open Vet J
Citation
Ariani, A., Khotimah, H., Sulistyarini, A., Daniaridevi, A.S. (2024) Centella asiatica extract ameliorates deoxygenation-induced neurological dysfunction in zebrafish larvae. Open veterinary journal. 14:115411601154-1160.
Abstract
Background Oxygen deprivation (OD) is a critical condition that can lead to brain damage and even death. Current hypoxia management approaches are limited in effectiveness. Centella asiatica (CA), known for its neuroprotective properties, offers a potential alternative for OD treatment.
Aims This study aims to investigate the neuroprotective effects of CA on the expression of brain-derived neurotrophic factor (BDNF) and vesicular glutamate transporter 1 (VGLUT1) in zebrafish larvae under oxygen-deficient conditions.
Methods Zebrafish embryos were subjected to low oxygen levels (1.5 mg/l) 0-2 hours post-fertilization (hpf) until 3 days post-fertilization (dpf), simulating the early stages of OD. Subsequent treatment involved varying concentrations of CA (1.25-5 µg/ml) up to 9 days post-fertilization. The expression levels of BDNF and VGLUT1 were measured using PCR methods. Statistical analysis was conducted using a two-way analysis of variance to evaluate the impact of CA on the expression of BDNF and VGLUT1 in zebrafish larvae aged 3 and 9 dpf in oxygen-deprived conditions.
Results CA significantly influenced the expression of BDNF and VGLUT1 under OD (p < 0.001). An increase in BDNF expression (p < 0.001) and a decrease in VGLUT1 (p < 0.01) were observed in zebrafish larvae experiencing OD and treated with CA. There was no significant difference in BDNF and VGLUT1 expression across age variations in zebrafish larvae at 3 dpf and 9 dpf in the treatment groups (p > 0.05). CA concentration of 2.5 µg/ml effectively enhanced BDNF and reduced VGLUT1 in 3-9 dpf zebrafish larvae.
Conclusion CA demonstrates potential as a neuroprotective agent, modulating increased BDNF expression and reduced VGLUT1 under OD conditions. These findings lay a foundation for further research in developing therapies for oxygen deficiency.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping