PUBLICATION
Dynamics of collagen oxidation and cross linking in regenerating and irreversibly infarcted myocardium
- Authors
- Akam-Baxter, E.A., Bergemann, D., Ridley, S.J., To, S., Andrea, B., Moon, B., Ma, H., Zhou, Y., Aguirre, A., Caravan, P., Gonzalez-Rosa, J.M., Sosnovik, D.E.
- ID
- ZDB-PUB-240611-4
- Date
- 2024
- Source
- Nature communications 15: 46484648 (Journal)
- Registered Authors
- Gonzalez-Rosa, Juan Manuel
- Keywords
- none
- MeSH Terms
-
- Cicatrix/metabolism
- Cicatrix/pathology
- Mice
- Disease Models, Animal
- Collagen*/metabolism
- Mice, Inbred C57BL
- Male
- Animals
- Regeneration*
- Myocardial Infarction*/metabolism
- Myocardial Infarction*/pathology
- Fluorescent Dyes/chemistry
- Zebrafish*
- Myocardium*/metabolism
- Myocardium*/pathology
- Oxidation-Reduction*
- PubMed
- 38858347 Full text @ Nat. Commun.
Citation
Akam-Baxter, E.A., Bergemann, D., Ridley, S.J., To, S., Andrea, B., Moon, B., Ma, H., Zhou, Y., Aguirre, A., Caravan, P., Gonzalez-Rosa, J.M., Sosnovik, D.E. (2024) Dynamics of collagen oxidation and cross linking in regenerating and irreversibly infarcted myocardium. Nature communications. 15:46484648.
Abstract
In mammalian hearts myocardial infarction produces a permanent collagen-rich scar. Conversely, in zebrafish a collagen-rich scar forms but is completely resorbed as the myocardium regenerates. The formation of cross-links in collagen hinders its degradation but cross-linking has not been well characterized in zebrafish hearts. Here, a library of fluorescent probes to quantify collagen oxidation, the first step in collagen cross-link (CCL) formation, was developed. Myocardial injury in mice or zebrafish resulted in similar dynamics of collagen oxidation in the myocardium in the first month after injury. However, during this time, mature CCLs such as pyridinoline and deoxypyridinoline developed in the murine infarcts but not in the zebrafish hearts. High levels of newly oxidized collagen were still seen in murine scars with mature CCLs. These data suggest that fibrogenesis remains dynamic, even in mature scars, and that the absence of mature CCLs in zebrafish hearts may facilitate their ability to regenerate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping