PUBLICATION
A β-catenin chromobody-based probe highlights endothelial maturation during vascular morphogenesis in vivo
- Authors
- Gauvrit, S., Zhao, S., Rothbauer, U., Stainier, D.Y.R.
- ID
- ZDB-PUB-240608-1
- Date
- 2024
- Source
- Development (Cambridge, England) 151(11): (Journal)
- Registered Authors
- Stainier, Didier
- Keywords
- Chromobody, Endothelium, Live imaging, Nanobody, Vascular morphogenesis, Zebrafish, β-catenin
- MeSH Terms
-
- Adherens Junctions/metabolism
- Animals
- Antigens, CD
- Cadherins*/genetics
- Cadherins*/metabolism
- Endothelial Cells/cytology
- Endothelial Cells/metabolism
- Endothelium, Vascular/cytology
- Endothelium, Vascular/metabolism
- Morphogenesis*
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- beta Catenin*/metabolism
- PubMed
- 38847494 Full text @ Development
Citation
Gauvrit, S., Zhao, S., Rothbauer, U., Stainier, D.Y.R. (2024) A β-catenin chromobody-based probe highlights endothelial maturation during vascular morphogenesis in vivo. Development (Cambridge, England). 151(11):.
Abstract
Visualization of protein dynamics is a crucial step in understanding cellular processes. Chromobodies, fluorescently labelled single-domain antibodies, have emerged as versatile probes for live cell imaging of endogenous proteins. However, how these chromobodies behave in vivo and how accurately they monitor tissue changes remain poorly explored. Here, we generated an endothelial-specific β-catenin chromobody-derived probe and analyzed its expression pattern during cardiovascular development in zebrafish. Using high-resolution confocal imaging, we show that the chromobody signal correlates with the localization of β-catenin in the nucleus and at cell-cell junctions, and thereby can be used to assess endothelial maturation. Loss of Cadherin 5 strongly affects the localization of the chromobody at the cell membrane, confirming the cadherin-based adherens junction role of β-catenin. Furthermore, using a genetic model to block blood flow, we observed that cell junctions are compromised in most endothelial cells but not in the endocardium, highlighting the heterogeneous response of the endothelium to the lack of blood flow. Overall, our data further expand the use of chromobodies for in vivo applications and illustrate their potential to monitor tissue morphogenesis at high resolution.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping