PUBLICATION
β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model
- Authors
- Zhang, P., Liu, N., Xue, M., Zhang, M., Xiao, Z., Xu, C., Fan, Y., Qiu, J., Zhang, Q., Zhou, Y.
- ID
- ZDB-PUB-240512-2
- Date
- 2024
- Source
- Animals : an open access journal from MDPI 14(9): (Journal)
- Registered Authors
- Keywords
- blood fat untargeted lipidomics, high-fat diet, lipid metabolism, non-alcoholic fatty liver disease model, zebrafish, ?-sitosterol
- MeSH Terms
- none
- PubMed
- 38731293 Full text @ Animals (Basel)
Citation
Zhang, P., Liu, N., Xue, M., Zhang, M., Xiao, Z., Xu, C., Fan, Y., Qiu, J., Zhang, Q., Zhou, Y. (2024) β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (Danio rerio) Model. Animals : an open access journal from MDPI. 14(9):.
Abstract
Objective Non-alcoholic fatty liver disease (NAFLD) is strongly associated with hyperlipidemia, which is closely related to high levels of sugar and fat. β-sitosterol is a natural product with significant hypolipidemic and cholesterol-lowering effects. However, the underlying mechanism of its action on aquatic products is not completely understood.
Methods A high-fat diet (HFD)-induced NAFLD zebrafish model was successfully established, and the anti-hyperlipidemic effect and potential mechanism of β-sitosterol were studied using oil red O staining, filipin staining, and lipid metabolomics.
Results β-sitosterol significantly reduced the accumulation of triglyceride, glucose, and cholesterol in the zebrafish model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differential lipid molecules in β-sitosterol mainly regulated the lipid metabolism and signal transduction function of the zebrafish model. β-sitosterol mainly affected steroid biosynthesis and steroid hormone biosynthesis in the zebrafish model. Compared with the HFD group, the addition of 500 mg/100 g of β-sitosterol significantly inhibited the expression of Ppar-γ and Rxr-α in the zebrafish model by at least 50% and 25%, respectively.
Conclusions β-sitosterol can reduce lipid accumulation in the zebrafish model of NAFLD by regulating lipid metabolism and signal transduction and inhibiting adipogenesis and lipid storage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping