PUBLICATION
Optimization of Zebrafish Larvae 6-OHDA Exposure for Neurotoxin Induced Dopaminergic Marker Reduction
- Authors
- Romero, A., Sanchez, A., Jones, J.D., Ledesma, K., El-Halawany, M.S., Hamouda, A.K., Bill, B.R.
- ID
- ZDB-PUB-240413-2
- Date
- 2024
- Source
- Zebrafish 21(4): 287-293 (Journal)
- Registered Authors
- Bill, Brent
- Keywords
- 6-OHDA, Parkinson's disease, RT-qPCR, superoxide dismutase, tyrosine hydroxylase, zebrafish
- MeSH Terms
-
- Disease Models, Animal
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Zebrafish*/genetics
- Dopaminergic Neurons*/drug effects
- Dopaminergic Neurons*/metabolism
- Protein Serine-Threonine Kinases
- Larva*/drug effects
- Larva*/genetics
- Larva*/growth & development
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- Animals
- Neurotoxins/toxicity
- Oxidopamine*/pharmacology
- PubMed
- 38608227 Full text @ Zebrafish
Citation
Romero, A., Sanchez, A., Jones, J.D., Ledesma, K., El-Halawany, M.S., Hamouda, A.K., Bill, B.R. (2024) Optimization of Zebrafish Larvae 6-OHDA Exposure for Neurotoxin Induced Dopaminergic Marker Reduction. Zebrafish. 21(4):287-293.
Abstract
Parkinson's disease (PD) is a neurodegenerative disorder that is clinically assessed by motor symptoms associated with the loss of midbrain dopaminergic neurons affecting the quality of life for over 8.5 million people worldwide. The neurotoxin 6-hydroxydopamine (6-OHDA) has been used to chemically induce a PD-like state in zebrafish larvae by several laboratories; however, highly variable concentration, methodology, and reagents have resulted in conflicting results suggesting a need to investigate these issues of reproducibility. We propose a protocol that addresses the differences in methodology and induces changes in 6 days postfertilization (dpf) larvae utilizing a 24-h exposure at 3 dpf with 30 μM 6-OHDA. Despite ∼50% lethality, no morphological or development differences in surviving fish are observed. Definition of our model is defined by downregulation of the expression of th1 by reverse transcriptase-quantitative polymerase chain reaction, a marker for dopaminergic neurons and a reduction in movement. Additionally, we observed a downregulation of pink1 and an upregulation of sod1 and sod2, indicators of mitochondrial dysfunction and response to reactive oxygen species, respectively.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping