PUBLICATION
A role for Retinoblastoma 1 in hindbrain morphogenesis by regulating GBX family
- Authors
- Zhao, S., Wang, C., Luo, H., Li, F., Wang, Q., Xu, J., Huang, Z., Liu, W., Zhang, W.
- ID
- ZDB-PUB-240404-11
- Date
- 2024
- Source
- Journal of genetics and genomics = Yi chuan xue bao 51(9): 900-910 (Journal)
- Registered Authors
- Huang, Zhibin, Wang, Qiang, Xu, Jin, Zhang, Wenqing
- Keywords
- Gbx1/Gbx2, Hindbrain, Proliferation, Rb1, Zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation/genetics
- Gene Expression Regulation, Developmental*/genetics
- Homeodomain Proteins*/genetics
- Homeodomain Proteins*/metabolism
- Mice
- Morphogenesis*/genetics
- Neurons/cytology
- Neurons/metabolism
- Retinoblastoma Protein/genetics
- Retinoblastoma Protein/metabolism
- Rhombencephalon*/embryology
- Rhombencephalon*/growth & development
- Rhombencephalon*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 38570112 Full text @ J. Genet. Genomics
Citation
Zhao, S., Wang, C., Luo, H., Li, F., Wang, Q., Xu, J., Huang, Z., Liu, W., Zhang, W. (2024) A role for Retinoblastoma 1 in hindbrain morphogenesis by regulating GBX family. Journal of genetics and genomics = Yi chuan xue bao. 51(9):900-910.
Abstract
The hindbrain, which develops from the anterior end of the neural tube expansion, can differentiate into the metencephalon and myelencephalon, with varying sizes and functions. The midbrain-hindbrain boundary (MHB) and hindbrain myelencephalon/ventral midline (HMVM) are known to be the source of the progenitors for the anterior hindbrain and myelencephalon, respectively. However, the molecular networks regulating hindbrain morphogenesis in these structures remain unclear. In this study, we show that rb1 is highly expressed at the MHB and HMVM in zebrafish. Knocking out rb1 in mice and zebrafish results in an enlarged hindbrain due to hindbrain neuronal hyperproliferation. Further study reveals that Rb1 controls the hindbrain morphogenesis by suppressing the expression of Gbx1/Gbx2, essential transcription factors for hindbrain development, through its binding to E2f3/Hdac1, respectively. Interestingly, we find that Gbx1 and Gbx2 were expressed in different types of hindbrain neurons, suggesting distinct roles in hindbrain morphogenesis. In summary, our study clarifies the specific role of RB1 in hindbrain neural cell proliferation and morphogenesis by regulating the E2f3-Gbx1 axis and the Hdac1-Gbx2 axis. These findings provide a research paradigm for exploring the differential proliferation of neurons in various brain regions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping