PUBLICATION
Projective light-sheet microscopy with flexible parameter selection
- Authors
- Chen, B., Chang, B.J., Daetwyler, S., Zhou, F., Sharma, S., Lee, D.M., Nayak, A., Noh, J., Dubrovinski, K., Chen, E.H., Glotzer, M., Fiolka, R.
- ID
- ZDB-PUB-240331-1
- Date
- 2024
- Source
- Nature communications 15: 27552755 (Journal)
- Registered Authors
- Chen, Elizabeth
- Keywords
- none
- MeSH Terms
-
- Brain/ultrastructure
- Zebrafish*
- Animals
- Larva
- Drosophila*
- Microscopy, Fluorescence/methods
- PubMed
- 38553438 Full text @ Nat. Commun.
Citation
Chen, B., Chang, B.J., Daetwyler, S., Zhou, F., Sharma, S., Lee, D.M., Nayak, A., Noh, J., Dubrovinski, K., Chen, E.H., Glotzer, M., Fiolka, R. (2024) Projective light-sheet microscopy with flexible parameter selection. Nature communications. 15:27552755.
Abstract
Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid projective light-sheet imaging with parameter selection (props) of imaging depth, position and viewing angle. This allows us to selectively image different sub-volumes of a sample, rapidly switch between them and exclude background fluorescence. Here we demonstrate the power of props by functional imaging within distinct regions of the zebrafish brain, monitoring calcium firing inside muscle cells of moving Drosophila larvae, super-resolution imaging of selected cell layers, and by optically unwrapping the curved surface of a Drosophila embryo. We anticipate that props will accelerate volumetric interrogation, ranging from subcellular to mesoscopic scales.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping