PUBLICATION

A Chemically Inducible Muscle Ablation System for the Zebrafish

Authors

Paulissen, E., Martin, B.L.

ID

ZDB-PUB-240305-1

Date

2024

Source

Zebrafish 21(3): 243-249 (Journal)

Registered Authors

Martin, Benjamin

Keywords

development, muscle, nitroreductase, regeneration, zebrafish

MeSH Terms

Metronidazole*/pharmacology
Muscle, Skeletal*/drug effects
Zebrafish*/genetics
Nitroreductases*/genetics
Nitroreductases*/metabolism
Regeneration/drug effects
Animals, Genetically Modified*
Animals

PubMed

38436568 Full text @ Zebrafish

Abstract

An effective method for tissue-specific ablation in zebrafish is the nitroreductase (NTR)/metronidazole (MTZ) system. Expressing bacterial NTR in the presence of nitroimidazole compounds causes apoptotic cell death, which can be useful for understanding many biological processes. However, this requires tissue-specific expression of the NTR enzyme, and many tissues have yet to be targeted with transgenic lines that express NTR. We generated a transgenic zebrafish line expressing NTR in differentiated skeletal muscle. Treatment of embryos with MTZ caused muscle specific cell ablation. We demonstrate this line can be used to monitor muscle regeneration in whole embryos and in transplanted transgenic cells.

Genes / Markers

Figures