PUBLICATION
Protocol for image-based small-molecule screen to identify neuroprotective compounds for dopaminergic neurons in zebrafish
- Authors
- Kim, G.J., Chen, M., Kwok, S., Guo, S.
- ID
- ZDB-PUB-240115-2
- Date
- 2024
- Source
- STAR protocols 5: 102837102837 (Journal)
- Registered Authors
- Guo, Su
- Keywords
- High-Throughput Screening, Model Organisms, Neuroscience
- MeSH Terms
-
- Animals
- Larva
- Dopaminergic Neurons*
- Image Processing, Computer-Assisted
- Zebrafish*
- Data Analysis
- PubMed
- 38217853 Full text @ STAR Protoc
Citation
Kim, G.J., Chen, M., Kwok, S., Guo, S. (2024) Protocol for image-based small-molecule screen to identify neuroprotective compounds for dopaminergic neurons in zebrafish. STAR protocols. 5:102837102837.
Abstract
Whole-organism-based screen holds promise for discovering biologically active compounds. However, high-content imaging is challenging due to the difficulty of positioning live animals and individual variability of neuron counts. Here, we present a protocol to identify neuroprotective compounds for dopaminergic neurons in zebrafish using an image-based small-molecule screen. We describe steps for raising larvae, agarose embedding, and treatment to induce neurodegeneration. We then detail procedures for live confocal imaging, image processing, and data analysis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2021).1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping