PUBLICATION
Antagonistic interactions safeguard mitotic propagation of genetic and epigenetic information in zebrafish
- Authors
- Lawir, D.F., Soza-Ried, C., Iwanami, N., Siamishi, I., Bylund, G.O., O Meara, C., Sikora, K., Kanzler, B., Johansson, E., Schorpp, M., Cauchy, P., Boehm, T.
- ID
- ZDB-PUB-240107-10
- Date
- 2024
- Source
- Communications biology 7: 3131 (Journal)
- Registered Authors
- Boehm, Tom, Schorpp, Michael
- Keywords
- none
- Datasets
- GEO:GSE181572, GEO:GSE181571, GEO:GSE181573
- MeSH Terms
-
- Alleles
- Animals
- DNA
- DNA Methylation*
- Epigenesis, Genetic
- Zebrafish*/genetics
- PubMed
- 38182651 Full text @ Commun Biol
Citation
Lawir, D.F., Soza-Ried, C., Iwanami, N., Siamishi, I., Bylund, G.O., O Meara, C., Sikora, K., Kanzler, B., Johansson, E., Schorpp, M., Cauchy, P., Boehm, T. (2024) Antagonistic interactions safeguard mitotic propagation of genetic and epigenetic information in zebrafish. Communications biology. 7:3131.
Abstract
The stability of cellular phenotypes in developing organisms depends on error-free transmission of epigenetic and genetic information during mitosis. Methylation of cytosine residues in genomic DNA is a key epigenetic mark that modulates gene expression and prevents genome instability. Here, we report on a genetic test of the relationship between DNA replication and methylation in the context of the developing vertebrate organism instead of cell lines. Our analysis is based on the identification of hypomorphic alleles of dnmt1, encoding the DNA maintenance methylase Dnmt1, and pole1, encoding the catalytic subunit of leading-strand DNA polymerase epsilon holoenzyme (Pole). Homozygous dnmt1 mutants exhibit genome-wide DNA hypomethylation, whereas the pole1 mutation is associated with increased DNA methylation levels. In dnmt1/pole1 double-mutant zebrafish larvae, DNA methylation levels are restored to near normal values, associated with partial rescue of mutant-associated transcriptional changes and phenotypes. Hence, a balancing antagonism between DNA replication and maintenance methylation buffers against replicative errors contributing to the robustness of vertebrate development.
Errata / Notes
Corrected by: ZDB-PUB-240301-17
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping