PUBLICATION
A Novel Anticancer Peptide Derived from Bryopsis plumosa Regulates Proliferation and Invasion in Non-Small Cell Lung Cancer Cells
- Authors
- Kim, H., Kim, H.T., Jung, S.H., Han, J.W., Jo, S., Kim, I.G., Kim, R.K., Kahm, Y.J., Choi, T.I., Kim, C.H., Lee, J.H.
- ID
- ZDB-PUB-231223-3
- Date
- 2023
- Source
- Marine drugs 21(12): (Journal)
- Registered Authors
- Keywords
- Bryopsis plumose, EMT, NSCLC, anticancer peptide, cancer
- MeSH Terms
-
- Animals
- Antineoplastic Agents*/pharmacology
- Antineoplastic Agents*/therapeutic use
- Carcinoma, Non-Small-Cell Lung*/pathology
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Epithelial-Mesenchymal Transition
- Humans
- Lung Neoplasms*/pathology
- Zebrafish
- PubMed
- 38132928 Full text @ Mar. Drugs
Citation
Kim, H., Kim, H.T., Jung, S.H., Han, J.W., Jo, S., Kim, I.G., Kim, R.K., Kahm, Y.J., Choi, T.I., Kim, C.H., Lee, J.H. (2023) A Novel Anticancer Peptide Derived from Bryopsis plumosa Regulates Proliferation and Invasion in Non-Small Cell Lung Cancer Cells. Marine drugs. 21(12):.
Abstract
The discovery of new highly effective anticancer drugs with few side effects is a challenge for drug development research. Natural or synthetic anticancer peptides (ACPs) represent a new generation of anticancer agents with high selectivity and specificity. The rapid emergence of chemoradiation-resistant lung cancer has necessitated the discovery of novel anticancer agents as alternatives to conventional therapeutics. In this study, we synthesized a peptide containing 22 amino acids and characterized it as a novel ACP (MP06) derived from green sea algae, Bryopsis plumosa. Using the ACP database, MP06 was predicted to possess an alpha-helical secondary structure and functionality. The anti-proliferative and apoptotic effects of the MP06, determined using the cytotoxicity assay and Annexin V/propidium iodide staining kit, were significantly higher in non-small-cell lung cancer (NSCLC) cells than in non-cancerous lung cells. We confirmed that MP06 suppressed cellular migration and invasion and inhibited the expression of N-cadherin and vimentin, the markers of epithelial-mesenchymal transition. Moreover, MP06 effectively reduced the metastasis of tumor xenografts in zebrafish embryos. In conclusion, we suggest considering MP06 as a novel candidate for the development of new anticancer drugs functioning via the ERK signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping