PUBLICATION
Circadian clock1a coordinates neutrophil recruitment via nfe212a/duox-reactive oxygen species pathway in zebrafish
- Authors
- Chen, A.Q., Xue, M., Qiu, C.Z., Zhang, H.Y., Zhou, R., Zhang, L., Yin, Z.J., Ren, D.L.
- ID
- ZDB-PUB-231002-132
- Date
- 2023
- Source
- Cell Reports 42: 113179113179 (Journal)
- Registered Authors
- Keywords
- CP: Immunology, CP: Molecular biology, ROS, circadian clock, neutrophil, nfe212a, zebrafish
- MeSH Terms
-
- Animals
- Circadian Rhythm*/genetics
- Neutrophil Infiltration
- Reactive Oxygen Species/metabolism
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 37756160 Full text @ Cell Rep.
Citation
Chen, A.Q., Xue, M., Qiu, C.Z., Zhang, H.Y., Zhou, R., Zhang, L., Yin, Z.J., Ren, D.L. (2023) Circadian clock1a coordinates neutrophil recruitment via nfe212a/duox-reactive oxygen species pathway in zebrafish. Cell Reports. 42:113179113179.
Abstract
Neutrophil recruitment to inflammatory sites appears to be an evolutionarily conserved strategy to fight against exogenous insults. However, the rhythmic characteristics and underlying mechanisms of neutrophil migration on a 24-h timescale are largely unknown. Using the advantage of in vivo imaging of zebrafish, this study explored how the circadian gene clock1a dynamically regulates the rhythmic recruitment of neutrophils to inflammatory challenges. We generated a clock1a mutant and found that neutrophil migration is significantly increased in caudal fin injury and lipopolysaccharide (LPS) injection. Transcriptome sequencing, chromatin immunoprecipitation (ChIP), and dual-luciferase reporting experiments suggest that the clock1a gene regulates neutrophil migration by coordinating the rhythmic expression of nfe212a and duox genes to control the reactive oxygen species (ROS) level. This study ultimately provides a visual model to expand the understanding of the rhythmic mechanisms of neutrophil recruitment on a circadian timescale in a diurnal organism from the perspective of ROS.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping