PUBLICATION
Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors
- Authors
- Dalle Carbonare, L., Minoia, A., Braggio, M., Bertacco, J., Piritore, F.C., Zouari, S., Vareschi, A., Elia, R., Vedovi, E., Scumà, C., Carlucci, M., Bhandary, L., Mottes, M., Romanelli, M.G., Valenti, M.T.
- ID
- ZDB-PUB-230910-57
- Date
- 2023
- Source
- International Journal of Molecular Sciences 24(17): (Journal)
- Registered Authors
- Keywords
- aging, circulating microRNAs, differentiation, miR-146b-5p
- MeSH Terms
-
- Aging/genetics
- Animals
- Exercise
- Female
- Humans
- Longevity
- Male
- MicroRNAs*/genetics
- Zebrafish*/genetics
- PubMed
- 37685971 Full text @ Int. J. Mol. Sci.
Citation
Dalle Carbonare, L., Minoia, A., Braggio, M., Bertacco, J., Piritore, F.C., Zouari, S., Vareschi, A., Elia, R., Vedovi, E., Scumà, C., Carlucci, M., Bhandary, L., Mottes, M., Romanelli, M.G., Valenti, M.T. (2023) Modulation of miR-146b Expression during Aging and the Impact of Physical Activity on Its Expression and Chondrogenic Progenitors. International Journal of Molecular Sciences. 24(17):.
Abstract
The finding of molecules associated with aging is important for the prevention of chronic degenerative diseases and for longevity strategies. MicroRNAs (miRNAs) are post-transcriptional regulators involved in many biological processes and miR-146b-5p has been shown to be involved in different degenerative diseases. However, miR-146b-5p modulation has not been evaluated in mesenchymal stem cells (MSCs) commitment or during aging. Therefore, the modulation of miR-146b-5p in the commitment and differentiation of mesenchymal cells as well as during maturation and aging in zebrafish model were analyzed. In addition, circulating miR-146b-5p was evaluated in human subjects at different age ranges. Thus, the role of physical activity in the modulation of miR-146b-5p was also investigated. To achieve these aims, RT (real-time)-PCR, Western blot, cell transfections, and three-dimensional (3D) culture techniques were applied. Our findings show that miR-146b-5p expression drives MSCs to adipogenic differentiation and increases during zebrafish maturation and aging. In addition, miR-146b-5p expression is higher in females compared to males and it is associated with the aging in humans. Interestingly, we also observed that the physical activity of walking downregulates circulating miR-146b-5p levels in human females and increases the number of chondroprogenitors. In conclusion, miR-146b-5p can be considered an age-related marker and can represent a useful marker for identifying strategies, such as physical activity, aimed at counteracting the degenerative processes of aging.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping