PUBLICATION

Zebrafish functional xenograft vasculature platform identifies PF-502 as a durable vasculature normalization drug

Authors: Zhong, J., Xiao, C., Chen, Q., Pan, X., Xu, T., Wang, Y., Hou, W., Liu, L., Cao, F., Wang, Y., Li, X., Zhou, L., Yang, H., Yang, Y., Zhao, C.
ID: ZDB-PUB-230908-66
Date: 2023
Source: iScience 26: 107734107734 (Journal)
Registered Authors
Keywords: Biological sciences, Cell biology, Molecular biology
MeSH Terms: none
PubMed: 37680473 Full text @ iScience

Abstract

Tumor vasculature often exhibits disorder and inefficiency. Vascular normalization offers potential for alleviating hypoxia and optimizing drug delivery in tumors. However, identifying effective agents is hindered by a lack of robust screening. We aimed to establish a comprehensive method using the zebrafish functional xenograft vasculature platform (zFXVP) to visualize and quantify tumor vasculature changes. Employing zFXVP, we systematically screened compounds, identifying PF-502 as a robust vascular normalization agent. Mechanistic studies showed PF-502 induces endothelial cell-cycle arrest, streamlines vasculature, and activates Notch1 signaling, enhancing stability and hemodynamics. In murine models, PF-502 exhibited pronounced vascular normalization and improved drug delivery at a sub-maximum tolerated dose. These findings highlight zFXVP's utility and suggest PF-502 as a promising adjunctive for vascular normalization in clinical settings.

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