PUBLICATION
Quantitative assessment of near-infrared fluorescent proteins
- Authors
- Zhang, H., Papadaki, S., Sun, X., Wang, X., Drobizhev, M., Yao, L., Rehbock, M., Köster, R.W., Wu, L., Namikawa, K., Piatkevich, K.D.
- ID
- ZDB-PUB-230905-57
- Date
- 2023
- Source
- Nature Methods 20(10): 1605-1616 (Journal)
- Registered Authors
- Köster, Reinhard W., Namikawa, Kazuhiko
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 37666982 Full text @ Nat. Methods
Citation
Zhang, H., Papadaki, S., Sun, X., Wang, X., Drobizhev, M., Yao, L., Rehbock, M., Köster, R.W., Wu, L., Namikawa, K., Piatkevich, K.D. (2023) Quantitative assessment of near-infrared fluorescent proteins. Nature Methods. 20(10):1605-1616.
Abstract
Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins (NIR FPs), which are rapidly becoming popular probes for a variety of imaging applications. However, the diversity of NIR FPs poses a challenge for end-users in choosing the optimal one for a given application. Here we conducted a systematic and quantitative assessment of intracellular brightness, photostability, oligomeric state, chemical stability and cytotoxicity of 22 NIR FPs in cultured mammalian cells and primary mouse neurons and identified a set of top-performing FPs including emiRFP670, miRFP680, miRFP713 and miRFP720, which can cover a majority of imaging applications. The top-performing proteins were further validated for in vivo imaging of neurons in Caenorhabditis elegans, zebrafish, and mice as well as in mice liver. We also assessed the applicability of the selected NIR FPs for multicolor imaging of fusions, expansion microscopy and two-photon imaging.
Genes / Markers
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Human Disease / Model
Sequence Targeting Reagents
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Engineered Foreign Genes
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