PUBLICATION

Development and repair of blood vessels in the zebrafish spinal cord

Authors
Ribeiro, A., Rebocho da Costa, M., de Sena-Tomás, C., Rodrigues, E.C., Quitéria, R., Maçarico, T., Rosa Santos, S.C., Saúde, L.
ID
ZDB-PUB-230810-55
Date
2023
Source
Open Biology   13: 230103230103 (Journal)
Registered Authors
Keywords
spinal cord, vascular development, vascular repair, zebrafish
MeSH Terms
  • Animals
  • Mammals
  • Spinal Cord Injuries*
  • Axons/physiology
  • Zebrafish*
PubMed
37553073 Full text @ Open Biol.
Abstract
The vascular system is inefficiently repaired after spinal cord injury (SCI) in mammals, resulting in secondary tissue damage and immune deregulation that contribute to the limited functional recovery. Unlike mammals, zebrafish can repair the spinal cord (SC) and restore motility, but the vascular response to injury has not been investigated. Here, we describe the zebrafish SC blood vasculature, starting in development with the initial vessel ingression in a body size-dependent manner, the acquisition of perivascular support and the establishment of ventral to dorsal blood circulation. The vascular organization grows in complexity and displays multiple barrier specializations in adulthood. After injury, vessels rapidly regrow into the lesion, preceding the glial bridge and axons. Vascular repair involves an early burst of angiogenesis that creates dysmorphic and leaky vessels. Dysfunctional vessels are later removed, as pericytes are recruited and the blood-SC barrier is re-established. This study demonstrates that zebrafish can successfully re-vascularize the spinal tissue, reinforcing the value of this organism as a regenerative model for SCI.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    is4TgTransgenic Insertion
      la2TgTransgenic Insertion
        mi2001TgTransgenic Insertion
          s896TgTransgenic Insertion
            s1010TgTransgenic Insertion
              w2
                		Point Mutation
                y1TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  No data available
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  CitrineEFGCitrine
                  EGFPEFGEGFP
                  GFPEFGGFP
                  mCherryEFGmCherry
                  1 - 4 of 4
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                  Mapping
                  No data available
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                  Citation
                  Ribeiro, A., Rebocho da Costa, M., de Sena-Tomás, C., Rodrigues, E.C., Quitéria, R., Maçarico, T., Rosa Santos, S.C., Saúde, L. (2023) Development and repair of blood vessels in the zebrafish spinal cord. Open Biology. 13:230103230103.