PUBLICATION
Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin
- Authors
- Pownall, M.E., Miao, L., Vejnar, C.E., M'Saad, O., Sherrard, A., Frederick, M.A., Benitez, M.D.J., Boswell, C.W., Zaret, K.S., Bewersdorf, J., Giraldez, A.J.
- ID
- ZDB-PUB-230719-28
- Date
- 2023
- Source
- Science (New York, N.Y.) 381: 9210092-100 (Journal)
- Registered Authors
- Giraldez, Antonio, Vejnar, Charles
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromatin*/chemistry
- Embryo, Nonmammalian
- Microscopy, Fluorescence*/methods
- Nanog Homeobox Protein/chemistry
- Nanog Homeobox Protein/metabolism
- Nucleosomes/chemistry
- RNA Polymerase II/chemistry
- RNA Polymerase II/metabolism
- Transcription, Genetic*
- Zebrafish
- Zygote*/metabolism
- PubMed
- 37410825 Full text @ Science
Citation
Pownall, M.E., Miao, L., Vejnar, C.E., M'Saad, O., Sherrard, A., Frederick, M.A., Benitez, M.D.J., Boswell, C.W., Zaret, K.S., Bewersdorf, J., Giraldez, A.J. (2023) Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin. Science (New York, N.Y.). 381:9210092-100.
Abstract
Nanoscale chromatin organization regulates gene expression. Although chromatin is notably reprogrammed during zygotic genome activation (ZGA), the organization of chromatin regulatory factors during this universal process remains unclear. In this work, we developed chromatin expansion microscopy (ChromExM) to visualize chromatin, transcription, and transcription factors in vivo. ChromExM of embryos during ZGA revealed how the pioneer factor Nanog interacts with nucleosomes and RNA polymerase II (Pol II), providing direct visualization of transcriptional elongation as string-like nanostructures. Blocking elongation led to more Pol II particles clustered around Nanog, with Pol II stalled at promoters and Nanog-bound enhancers. This led to a new model termed "kiss and kick", in which enhancer-promoter contacts are transient and released by transcriptional elongation. Our results demonstrate that ChromExM is broadly applicable to study nanoscale nuclear organization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping