PUBLICATION
Ripply suppresses Tbx6 to induce dynamic-to-static conversion in somite segmentation
- Authors
- Yabe, T., Uriu, K., Takada, S.
- ID
- ZDB-PUB-230414-75
- Date
- 2023
- Source
- Nature communications 14: 21152115 (Journal)
- Registered Authors
- Takada, Shinji
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Expression Regulation, Developmental
- Mesoderm/metabolism
- Somites*/metabolism
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 37055428 Full text @ Nat. Commun.
Citation
Yabe, T., Uriu, K., Takada, S. (2023) Ripply suppresses Tbx6 to induce dynamic-to-static conversion in somite segmentation. Nature communications. 14:21152115.
Abstract
The metameric pattern of somites is created based on oscillatory expression of clock genes in presomitic mesoderm. However, the mechanism for converting the dynamic oscillation to a static pattern of somites is still unclear. Here, we provide evidence that Ripply/Tbx6 machinery is a key regulator of this conversion. Ripply1/Ripply2-mediated removal of Tbx6 protein defines somite boundary and also leads to cessation of clock gene expression in zebrafish embryos. On the other hand, activation of ripply1/ripply2 mRNA and protein expression is periodically regulated by clock oscillation in conjunction with an Erk signaling gradient. Whereas Ripply protein decreases rapidly in embryos, Ripply-triggered Tbx6 suppression persists long enough to complete somite boundary formation. Mathematical modeling shows that a molecular network based on results of this study can reproduce dynamic-to-static conversion in somitogenesis. Furthermore, simulations with this model suggest that sustained suppression of Tbx6 caused by Ripply is crucial in this conversion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping