PUBLICATION
The non-canonical Wnt receptor Ror2 is required for cartilage cell polarity and morphogenesis of the craniofacial skeleton in zebrafish
- Authors
- Dranow, D.B., Le Pabic, P., Schilling, T.F.
- ID
- ZDB-PUB-230412-49
- Date
- 2023
- Source
- Development (Cambridge, England) 150(8): (Journal)
- Registered Authors
- Schilling, Tom
- Keywords
- Cartilage, Cell polarity, Non-canonical Wnt, Ror2, Zebrafish
- MeSH Terms
-
- Animals
- Bone and Bones/metabolism
- Cartilage/metabolism
- Cell Polarity/genetics
- Chondrocytes*/metabolism
- Morphogenesis/genetics
- Receptor Tyrosine Kinase-like Orphan Receptors/genetics
- Receptor Tyrosine Kinase-like Orphan Receptors/metabolism
- Receptors, Wnt/metabolism
- Wnt Proteins/genetics
- Wnt Proteins/metabolism
- Wnt Signaling Pathway/genetics
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins
- PubMed
- 37039156 Full text @ Development
Citation
Dranow, D.B., Le Pabic, P., Schilling, T.F. (2023) The non-canonical Wnt receptor Ror2 is required for cartilage cell polarity and morphogenesis of the craniofacial skeleton in zebrafish. Development (Cambridge, England). 150(8):.
Abstract
Non-canonical / β-catenin-independent Wnt signaling plays critical roles in tissue / cell polarity in epithelia, but its functions have been less well studied in mesenchymal tissues, such as the skeleton. Mutations in non-canonical Wnt signaling pathway genes cause human skeletal diseases such as Robinow syndrome and Brachydactyly Type B1, which disrupt bone growth throughout the endochondral skeleton. Ror2 is one of several non-canonical Wnt receptor/co-receptors. Here we show that ror2-/- mutant zebrafish have craniofacial skeletal defects, including disruptions of chondrocyte polarity. ror1-/- mutants appear phenotypically wild type, but loss of both ror1 and ror2 leads to more severe cartilage defects, indicating partial redundancy. Skeletal defects in ror1/2 double mutants resemble those of wnt5b-/- mutants, suggesting that Wnt5b is the primary Ror ligand in zebrafish. Surprisingly, the proline-rich domain of Ror2, but not its kinase domain, is required to rescue its function in mosaic transgenic experiments in ror2-/- mutants. These results suggest that endochondral bone defects in ROR-related human syndromes reflect defects in cartilage polarity and morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping