PUBLICATION
Annexin-A5 and annexin-A6 silencing prevents metastasis of breast cancer cells in zebrafish
- Authors
- Gounou, C., Bouvet, F., Liet, B., Prouzet-Mauléon, V., d'Agata, L., Harté, E., Argoul, F., Siegfried, G., Iggo, R., Khatib, A.M., Bouter, A.
- ID
- ZDB-PUB-230324-47
- Date
- 2023
- Source
- Biology of the cell 115(6): e202200110 (Journal)
- Registered Authors
- Keywords
- Cancer, annexin-A5, annexin-A6, annexins, invasion, membrane repair, metastasis
- MeSH Terms
-
- Extracellular Matrix/metabolism
- Annexin A5/genetics
- Annexin A5/metabolism
- Cell Membrane/metabolism
- Zebrafish*/metabolism
- Neoplasms*/metabolism
- Animals
- Annexin A6/genetics
- Annexin A6/metabolism
- PubMed
- 36958013 Full text @ Biol. Cell
Citation
Gounou, C., Bouvet, F., Liet, B., Prouzet-Mauléon, V., d'Agata, L., Harté, E., Argoul, F., Siegfried, G., Iggo, R., Khatib, A.M., Bouter, A. (2023) Annexin-A5 and annexin-A6 silencing prevents metastasis of breast cancer cells in zebrafish. Biology of the cell. 115(6):e202200110.
Abstract
Background information During tumor invasion and metastasis processes, cancer cells are exposed to major compressive and shearing forces, due to their migration through extracellular matrix, dense cell areas and complex fluids, which may lead to numerous plasma membrane damages RESULTS: : Cancer cells may survive to these mechanical stresses thanks to an efficient membrane repair machinery. Consequently, this machinery may constitute a relevant target to inhibit cancer cell dissemination.
Conclusions We show here that annexin-A5 (ANXA5) and ANXA6 participate in membrane repair of MDA-MB-231 cells, a highly invasive triple-negative breast cancer cell line. These crucial components of the membrane repair machinery are substantially expressed in breast cancer cells in correlation with their invasive properties.
Significance In addition, high expression of ANXA5 and ANXA6 predict poor prognosis in high-grade lung, gastric and breast cancers. In zebrafish, the genetic inhibition of ANXA5 and ANXA6 leads to drastic reduction of tumor cell dissemination. We conclude that the inhibition of ANXA5 and ANXA6 prevents membrane repair in cancer cells, which are thus unable to survive to membrane damage during metastasis. This result opens a new therapeutic strategy based on targeting membrane repair machinery to inhibit tumor invasion and metastasis. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping