PUBLICATION
In vivo investigation of mitochondria in lateral line afferent neurons and hair cells
- Authors
- Wong, H.C., Lukasz, D., Drerup, C.M., Kindt, K.S.
- ID
- ZDB-PUB-230323-41
- Date
- 2023
- Source
- Hearing Research 431: 108740108740 (Review)
- Registered Authors
- Drerup, Katie (Catherine), Kindt, Katie, Wong, Hiu-Tung Candy
- Keywords
- Afferent neurons, Hair cells, Hearing and balance, In vivo imaging, Lateral line, Mitochondria, Zebrafish
- MeSH Terms
-
- Mitochondria
- Synaptic Transmission
- Neurons, Afferent/physiology
- Hair Cells, Auditory/physiology
- Zebrafish
- Animals
- Lateral Line System*
- PubMed
- 36948126 Full text @ Hear. Res.
Citation
Wong, H.C., Lukasz, D., Drerup, C.M., Kindt, K.S. (2023) In vivo investigation of mitochondria in lateral line afferent neurons and hair cells. Hearing Research. 431:108740108740.
Abstract
To process sensory stimuli, intense energy demands are placed on hair cells and primary afferents. Hair cells must both mechanotransduce and maintain pools of synaptic vesicles for neurotransmission. Furthermore, both hair cells and afferent neurons must continually maintain a polarized membrane to propagate sensory information. These processes are energy demanding and therefore both cell types are critically reliant on mitochondrial health and function for their activity and maintenance. Based on these demands, it is not surprising that deficits in mitochondrial health can negatively impact the auditory and vestibular systems. In this review, we reflect on how mitochondrial function and dysfunction are implicated in hair cell-mediated sensory system biology. Specifically, we focus on live imaging approaches that have been applied to study mitochondria using the zebrafish lateral-line system. We highlight the fluorescent dyes and genetically encoded biosensors that have been used to study mitochondria in lateral-line hair cells and afferent neurons. We then describe the impact this in vivo work has had on the field of mitochondrial biology as well as the relationship between mitochondria and sensory system development, function, and survival. Finally, we delineate the areas in need of further exploration. This includes in vivo analyses of mitochondrial dynamics and biogenesis, which will round out our understanding of mitochondrial biology in this sensitive sensory system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping