PUBLICATION
Single-cell sequencing reveals the evolution of immune molecules across multiple vertebrate species
- Authors
- Jiao, A., Zhang, C., Wang, X., Sun, L., Liu, H., Su, Y., Lei, L., Li, W., Ding, R., Ding, C., Dou, M., Tian, P., Sun, C., Yang, X., Zhang, L., Zhang, B.
- ID
- ZDB-PUB-230306-33
- Date
- 2023
- Source
- Journal of advanced research 55: 73-87 (Journal)
- Registered Authors
- Keywords
- Across species, Adaptive immunity, Evolution, Innate immunity, ScRNA-Seq
- Datasets
- GEO:GSE186158
- MeSH Terms
-
- Adaptive Immunity*/genetics
- Animals
- Mice
- Swine
- Humans
- Macrophages
- Zebrafish/genetics
- Transcriptome*
- Immunity, Innate*/genetics
- PubMed
- 36871615 Full text @ J Adv Res
Citation
Jiao, A., Zhang, C., Wang, X., Sun, L., Liu, H., Su, Y., Lei, L., Li, W., Ding, R., Ding, C., Dou, M., Tian, P., Sun, C., Yang, X., Zhang, L., Zhang, B. (2023) Single-cell sequencing reveals the evolution of immune molecules across multiple vertebrate species. Journal of advanced research. 55:73-87.
Abstract
Introduction Both innate and adaptive immune system undergo evolution from low to high vertebrates. Due to the limitation of conventional approaches in identifying broader spectrum of immune cells and molecules from various vertebrates, it remains unclear how immune molecules evolve among vertebrates.
Objectives Here, we utilized carry out comparative transcriptome analysis in various immune cells across seven vertebrate species.
Methods Single-cell RNA sequencing (scRNA-seq).
Results We uncovered both conserved and species-specific profiling of gene expression in innate and adaptive immunity. Macrophages exhibited highly-diversified genes and developed sophisticated molecular signaling networks along with evolution, indicating effective and versatile functions in higher species. In contrast, B cells conservatively evolved with less differentially-expressed genes in analyzed species. Interestingly, T cells represented a dominant immune cell populations in all species and unique T cell populations were identified in zebrafish and pig. We also revealed compensatory TCR cascade components utilized by different species. Inter-species comparison of core gene programs demonstrated mouse species has the highest similarity in immune transcriptomes to human.
Conclusions Therefore, our comparative study reveals gene transcription characteristics across multiple vertebrate species during the evolution of immune system, providing insights for species-specific immunity as well as the translation of animal studies to human physiology and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping