PUBLICATION
Zn-Al layered double hydroxides induce embryo malformations and impair locomotion behavior in Danio rerio
- Authors
- Carneiro, D., Damasceno, É.P., Ferreira, V., Charlie-Silva, I., Tedim, J., Maia, F., Loureiro, S., Martins, R., Pavlaki, M.D.
- ID
- ZDB-PUB-230226-34
- Date
- 2023
- Source
- NanoImpact 30: 100457 (Journal)
- Registered Authors
- Loureiro, Susana
- Keywords
- Embryotoxicity, Engineered nanoclays, Stimuli-responsive nanomaterials, Sub-lethal effects, Zebrafish
- MeSH Terms
-
- Animals
- Ecosystem
- Embryo, Nonmammalian*/abnormalities
- Locomotion
- Zebrafish*/abnormalities
- Zinc/pharmacology
- PubMed
- 36828229 Full text @ NanoImpact
Citation
Carneiro, D., Damasceno, É.P., Ferreira, V., Charlie-Silva, I., Tedim, J., Maia, F., Loureiro, S., Martins, R., Pavlaki, M.D. (2023) Zn-Al layered double hydroxides induce embryo malformations and impair locomotion behavior in Danio rerio. NanoImpact. 30:100457.
Abstract
Layered double hydroxides (LDHs) are stimuli-responsive anionic nanoclays. The vast possibilities of using LDHs can lead to their existence in the ecosystem, raising a question of potential ecological concern. However, little is known about the effect of these nanomaterials on freshwater organisms. The present study aimed to assess the ecotoxicological effects of Zinc-Aluminium LDH-nitrate (ZnAl LDH-NO3) in zebrafish (Danio rerio) early life stages. The endpoints measured were mortality, malformations and hatching rate after exposure of D. rerio embryos and larvae to ZnAl LDH-NO3 following the OECD 236 guideline. The behavioral, biochemical (markers of oxidative stress and neurotoxicity), and molecular (at DNA level) alterations were also assessed using sub-lethal concentrations. No observable acute effects were detected up to 415.2 mg LDH/L while the 96 h-LC50 was estimated as 559.9 mg/L. Tested LDH caused malformations in D. rerio embryos, such as pericardial edema, incomplete yolk sac absorption and tail deformities (96 h-EC50 = 172.4 mg/L). During the dark periods, the locomotor behavior in zebrafish larvae was affected upon ZnAl LDH-NO3 exposure. However, no significant biochemical and molecular changes were recorded. The present findings suggest that ZnAl LDH-NO3 can be regarded as a non-toxic nanomaterial towards D. rerio (E/LC50 > > 100 mg/L) although impairment of the locomotion behavior on zebrafish embryos can be expected at concentrations below 100 mg/L.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping