PUBLICATION
Specificity of the innate immune responses to different classes of non-tuberculous mycobacteria
- Authors
- Hu, W., Koch, B.E.V., Lamers, G.E.M., Forn-Cuní, G., Spaink, H.P.
- ID
- ZDB-PUB-230207-15
- Date
- 2023
- Source
- Frontiers in immunology 13: 10754731075473 (Journal)
- Registered Authors
- Koch, Bjorn, Spaink, Herman P.
- Keywords
- Tlr2, leukocyte migration, metabolism, nontuberculous mycobacterium, zebrafish
- Datasets
- GEO:GSE218892
- MeSH Terms
-
- Animals
- Immunity, Innate
- Larva
- Mycobacterium Infections, Nontuberculous*
- Nontuberculous Mycobacteria*
- Toll-Like Receptor 2
- Zebrafish
- PubMed
- 36741407 Full text @ Front Immunol
Citation
Hu, W., Koch, B.E.V., Lamers, G.E.M., Forn-Cuní, G., Spaink, H.P. (2023) Specificity of the innate immune responses to different classes of non-tuberculous mycobacteria. Frontiers in immunology. 13:10754731075473.
Abstract
Mycobacterium avium is the most common nontuberculous mycobacterium (NTM) species causing infectious disease. Here, we characterized a M. avium infection model in zebrafish larvae, and compared it to M. marinum infection, a model of tuberculosis. M. avium bacteria are efficiently phagocytosed and frequently induce granuloma-like structures in zebrafish larvae. Although macrophages can respond to both mycobacterial infections, their migration speed is faster in infections caused by M. marinum. Tlr2 is conservatively involved in most aspects of the defense against both mycobacterial infections. However, Tlr2 has a function in the migration speed of macrophages and neutrophils to infection sites with M. marinum that is not observed with M. avium. Using RNAseq analysis, we found a distinct transcriptome response in cytokine-cytokine receptor interaction for M. avium and M. marinum infection. In addition, we found differences in gene expression in metabolic pathways, phagosome formation, matrix remodeling, and apoptosis in response to these mycobacterial infections. In conclusion, we characterized a new M. avium infection model in zebrafish that can be further used in studying pathological mechanisms for NTM-caused diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping